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© 1989 Oxford University Press

research-article

Sensitivity of rat and mouse peripheral blood lymphocytes to BaP adduction and SCE formation

Andrew D. Kligerman , Stephen Nesnow , Gregory L. Erexson 1, Karen Earley 2 and Ramesh C. Gupta 2

1Environmental Health Research and Testing PO Box 12199, Research Triangle Park, NC 27709
2Department of Pharmacology, Baylor College of Medicine Houston, TX 77030, USA
Genetic Toxicology Division, Health Effects Research Laboratory, US Environmental Protection Agency Research Triangle Park, NC 27711

Both mice and rats were injected i.p. with doses of benzo[a]pyrene (BaP) ranging from 10 to 100 mg/kg to and compare species sensitivity to and the relationship between sister chromatid exchange (SCE) induction and DNA adduct formation. Twenty-four hours after injection, blood was removed by cnrdiac puncture and the peripheral blood lymphocytes (PBLs) were analyzed for both DNA adduct formation by 32P-postlabeling and SCE induction following lymphocyte culture. BaP induced similar, but not identical, SCE dose-response curves for each species. After BaP administration, the major DNA adduct, N2-[10ß-(7ß, 8{alpha}, 9{alpha}-trihydroxy-7, 8, 9, 10-tetrahydrobenzo[a]pyrene)y1]deoxy-guanosine (BPDEI-dGuo), was ~ 10-fold more prevalent in the PBLs of the mouse than those of the rat. Thus, for equivalent amounts of BPDEI-dGuo, a greater number of SCEs are induced in the rat than the mouse.


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