Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (20)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Schrenk, D.
Right arrow Articles by Dekant, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schrenk, D.
Right arrow Articles by Dekant, W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1989 Oxford University Press

other

Covalent binding of hexachlorobutadiene metabolites to renal and hepatic mitochondrial DNA

D. Schrenk 1 and W. Dekant 2

Institut fur Toxikologie, Universitat Wurzburg Versbacher Strasse 9, D-8700 Wurzburg, FRG

2To whom correspondence should be sent

The covalent binding of [14C]hexachlorobutadiene (HCBD) metabolites to DNA was investigated in mouse liver and kidney after application of a single oral dose of 30 mg/kg. A low level of binding of [14C]HCBD metabolites to nuclear DNA (nDNA) from kidney with a covalent binding index (CBI) of 27 was detectable, whereas nDNA from liver did not retain radioactivity significantly exceeding background activity (background CBInDNAna = 6 and CBImtDNA = 60). Considerably higher binding of metabolites to mitochondrial DNA (mtDNA) from liver (CBI = 500) and kidney (CBI = 7500) could be demonstrated. Enzymatic hydrolysis followed by HPLC fractionation of mtDNA from mouse kidney revealed the presence of three radioactive compounds which may represent DNA bases altered by HCBD metabolites. The observed binding of HCBD metabolites to DNA constituents in vivo suggests that genotoxic mechanisms are operative in initiating HCBD nephrocarcinogenesis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Toxicol PatholHome page
Y. Nakagawa, Y. Kitahori, M. Cho, N. Konishi, K.-I. Tsumatani, S. Ozono, E. Okajima, Y. Hirao, and Y. Hiasa
Effect of Hexachloro-1,3-butadiene on Renal Carcinogenesis in Male Rats Pretreated with N-Ethyl-N-hydroxyethylnitrosamine
Toxicol Pathol, May 1, 1998; 26(3): 361 - 366.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.