© 1989 Oxford University Press
other |
SHORT COMMUNICATION: Comparison of 5-bromo-2-deoxyuridine and [3H]thymidine for studies of hepatocellular proliferation in rodents
1Departments of Biochemical Toxicology, Lilly Research Laboratories Eli Lilly and Company, Greenfield, IN 46140, USA
2Departments of Pathology, Lilly Research Laboratories Eli Lilly and Company, Greenfield, IN 46140, USA
3To whom correspondence should be addressed
Hepatocyte replication traditionally has been studied by [3H]thymidine (TdR) incorporation into DNA, and more recently using incorporation of 5-bromo-2-deoxyuridine (BUdR), a synthetic analog of thymidine which is measured by immunohistochemistry. In studies to compare TdR and BUdR, mice were given the peroxisome proliferator [4-chloro-6-(2, 3-xylidino)-2-pyrimidinylthio]acetic acid (WY-14643) in the diet (0.1%) for 5 days and either TdR (1µg) or BUdR (100 mg/kg) on days 2–5. The labeling index (LI) for hepatocytes of WY-14643-treated mice was 4.7% with BUdR and 5.2% with TdR. The LI for control mice was 0.3–0.4% with either label. Partially hepatectomized rats given TdR had a mean LI of 30.0 versus 32.0% in rats labeled with BUdR. Sham-operated controls given TdR had a mean LI of 0.2% and BUdR controls had a mean LI of 0.1%. Hepatectomized rats given TdR and BUdR simultaneously had an LI of 20.1% for TdR and 22.4% for BUdR. In these rats, 94.1% of the labeled cells contained both markers, whereas 1.9% had only TdR and 4.0% had only BUdR. Comparable labeling indices using either TdR or BUdR indicate that the analogs may be used interchangeably in short-term in vivo studies of liver cell proliferation.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. M. Brennan, E. M. Boncavage-Hennessey, M. J. Wolfe, M. W. Toussaint, W. E. Dennis, A. B. Rosencrance, and H. S. Gardner JR An In Vivo Method for Using 5-Bromo-2'-deoxyuridine (BrdU) as a Marker of Chemically-Induced Hepatocellular Proliferation in the Japanese Medaka (Oryzias latipes) Toxicol Pathol, April 1, 2001; 29(3): 387 - 397. [Abstract] [PDF]
|
|
|
|
 |
|
 L. Lambotte, A. Saliez, S. Triest, E. M. Tagliaferri, A. P. Barker, and A. G. Baranski Control of rate and extent of the proliferative response after partial hepatectomy Am J Physiol Gastrointest Liver Physiol, October 1, 1997; 273(4): G905 - G912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. B. Lappin and R. A. Roth Hypertrophy and Prolonged DNA Synthesis in Smooth Muscle Cells Characterize Pulmonary Arterial Wall Thickening After Monocrotaline Pyrrole Administration to Rats Toxicol Pathol, July 1, 1997; 25(4): 372 - 380. [Abstract] [PDF]
|
|
|
|
|
|
N. D. Lalwani, L. A. Dethloff, J. R. Haskins, D. G. Robertson, and F. A. De La Iglesia Increased Nuclear Ploidy, Not Cell Proliferation, Is Sustained in the Peroxisome Proliferator-Treated Rat Liver Toxicol Pathol, March 1, 1997; 25(2): 165 - 176. [Abstract] [PDF]
|
|
|
|
|
|
W. Thamavit, D. Tiwawech, M. A. Moore, N. Ito, and T. Shirai Equivocal Evidence of Complete Carcinogenicity after Repeated Infection of Syrian Hamsters with Opisthorchis viverrini Toxicol Pathol, July 1, 1996; 24(4): 493 - 497. [Abstract] [PDF]
|
|
|
|
|
|
T. L. Goldsworthy, R. Fransson-Steen, and R. R. Maronpot Importance of and Approaches to Quantification of Hepatocyte Apoptosis Toxicol Pathol, January 1, 1996; 24(1): 24 - 35. [Abstract] [PDF]
|
|