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© 1990 Oxford University Press

other

Evidence for spontaneous conversion of Mex– to Mex+in human lymphoblastoid cells

Izumi Arita, Akira Fujimori, Hiraku Takebe and Kouichi Tatsumi 1

Department of molecular Oncology, Faculty of Medicine, Kyoto University Sakyo, Kyoto 606, Japan

1To whom correspondence should be addressed

A series of human lymphoblastoid cell lines (LCLs) called Mex– were defined by Sklar and Strauss on the basis of their inability to remove O6-methylguanine from DNA. Instability of Mex– has previously been shown as a population phenotype of LCLs. We examined whether Mex– as a cellular phenotype is spontaneously convertible or not. At the population doubling number (PDN) 23 after recloning, two out of 15 independent sibcultures derived from a Mex– LCL, AT1-1, were found to contain a small fraction of Mex+ cells after treatment with 1-(4-amino-2-methyl-5-pyr-imidinyl)methyl-3-(2-chloroethyl)-3-nitrosourea (ACNU). Three Mex+ subclones were identified without exposure to ACNU among 486 subclones from replica plating of an expanded Mex– clone (PDN30). The rate of spontaneous conversion was estimated to be in the range of 10–8–10–7 per cell per generation by the fluctuation analyses on two Mex– subclones. These results strongly support the hypothesis that Mex– as a cellular phenotype is spontaneously convertible to Mex+.


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