Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (53)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Prevost, V.
Right arrow Articles by Tannenbaum, S.R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Prevost, V.
Right arrow Articles by Tannenbaum, S.R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1990 Oxford University Press

other

The determination of urinary 3-methyladenine by immnunoaffinity chromatography-monoclonal antibody-based ELISA: use in human biomonitoring studies

V. Prevost, D.E.G. Shuker 2, H. Bartsch, R. Pastorelli 1, W.G. Stillwell 1, L.J. Trudel 1 and S.R. Tannenbaum 1 2

International Agency for Research on Cancer 150 Cours Albert Thomas, 69372 Lyon Cedex 08, France
1Department of Chemistry and Division of Toxicology, Massachusetts Institute of Technology Cambndge, MA 02139, USA

2To whom correspondence should be addresed

A mouse monoclonal antibody (Mab) was prepared which showed high specificity for a potential marker of exposure to methylating carcinogens such as 3-methyladenine (3-MeAde). In a low-temperature (4°C) ELISA a linear calibration curve was obtained between 3 and 50 fmol/well. In combination with an immunoaffinity (IA) column prepared from a 3-MeAde rabbit antiserum, the ELISA was used to determine 3-MeAde in urine. The IA-ELISA method was validated by comparison with results obtained by an IA-GC-MS method. The effect of consuming a low 3-MeAde diet on urinary 3-MeAde excretion was investigated in a human volunteer. Urine collected during a ‘nornml’ diet exhibited the characteristic variation in 3-MeAde levels previously observed (9.5 µg/24 h, SD = 4.4, n = 5). In contrast, 3-MeAde excretion was markedly lower and less variable on days when the diet was closely controlled (0.63 µg/ h, SD = 0.08, n = 3). Dietary intake of 3-MeAde on the latter days was between 0.37 and 0.43 µg/day, indicating that most, if not all, of the 3-MeAde seen in previous experiments was derived from the diet. The origin of dietary 3-MeAde is not known, but may be related to fumigant use. Dietary manipulation affords the possibility of carrying out model studies, in volunteers, on 3-MeAde intake and formation in vivo.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Exp ToxicolHome page
V. Niot-Mansart, A. Muhamedi, and J.-P. Arnould
A competitive ELISA detecting 7-methylguanosine adduct induced by N-nitrosodimethylamine exposure
Human and Experimental Toxicology, February 1, 2005; 24(2): 89 - 94.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.