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© 1990 Oxford University Press

other

A new sensitvie fluorometric assay for the metabolism of (–)-7,8-dihydroxy-7,8-dihydrobenzo[{alpha}]pyrene by human hair follicles

Kroum Alexandrov 1, Margarita Rojas, Mark Goldberg 2, Anne-Maria Camus and Helmut Bartsch

Unit of Environmental Carcinogens and Host Factors, International Agency for Research on Cancer 150 cours Albert Thomas, 69372 Lyon Cedex 08, France
1 Visiting scientist on leave form CNRS, BP 2, 94802 Villejuif, France

A new sensitive fluorometric assay was established to measure the stereospecific formation of benzo [{alpha}]pyrene tetrols formed after cytochrome P450-dependent metabolism of (–)-7,8-dihydroxy-7,8-dihydrobenzo by human hair follicles. This simple assay requires three human hair follicles and a low (0.5–2.0 µM) substrate concentration and has a limit of detection of ~0.3 fmol of tetrols. Freshly isolated human hair follicles from 20 adult volunteers (10 non-smokers and 10 smokers) were assayed. While intersubject and seasonal variations were observed, the assay was found to be reproducible for a given subject. This rapid and non-invasive assay provides a new means for metabolic phenotyping of human subjects for their capacity to metabolize (–)-7,8-dihydroxy-7,8-dihydrobenzo [{alpha}]pyrene to its carcino genic form (+)anti-benzo[{alpha}] diolepoxide.


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