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© 1990 Oxford University Press

other

Solid-phase synthesis of oligodeoxynucleotides containing O6-alkylguanine

Clive A. Smith 1, Yao-Zhong Xu 2 and Peter F. Swann

Department of Biochemistry, University College and Middlesex School of Medicine Gower St. London WCIE 6BT, UK

1Present address: Cruachem Ltd, West of Scotland Science Park, Acre, Road, Glasgow, UK

2Permanent address: Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Lu, Shanghai 200032, China

Practical methods for the routine solid-phase synthesis of oligodeoxynucleotides containing O6-alkylguanine are described. It is shown that if the 2-amino group of the alkylated base is protected with a phenylacetyl group, and a mixture of nitrobenzaldoximate ions and ammonia used to remove the protecting groups from the oligomer at the end of the synthesis, there is negligible formation of 2,6-diainino-purine and that after chromatography pure oligomers are obtained.


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