© 1990 Oxford University Press
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A reassessment of methylcholanthrene transformation in the C3H10T1/2 cell culture system
1Molecular Biology Institute UCLA Center for the Health Sciences, University of California at Los Angeles, Los Angeles, CA 90024-1786, USA
2Department of Biological Chemistry UCLA Center for the Health Sciences, University of California at Los Angeles, Los Angeles, CA 90024-1786, USA
3Laboratory of Biomedical and Environmental Sciences UCLA Center for the Health Sciences, University of California at Los Angeles, Los Angeles, CA 90024-1786, USA
We previously demonstrated that four tumorigenic methylcholanthrene (MCA) transformed cell lines derived from C3H10T1/2 cells each contain a common G34 – T nucleotide alteration in the c-Ki-ras gene. In contrast, a non-twnorigenic MCA transformant does not contain this mutation. We have now examined 75 newly isolated MCA transformants of C3H10T1/2 cells for their degree of morphological transfonnation, the presence of the c-Ki-ras G34 – T mutation, colony formation in soft agar, and tumorigenlclty in nude mice. Although many of these new MCA transformants exhibit morphological characteristics indistinguishable from previously isolated tumorigenic MCA transformants, none contain the G34 – T mutation in the c-Ki-ras gene. Only one newly isolated MCA transformant can grow in soft agar. Of 14 tested, none of the new MCA C3H10T1/2 transformants are tumorigenic in nude mice.