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© 1990 Oxford University Press

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Liver hyperplasia is not necessarily associated with increased expression of c-fos and c-myc mRNA

P. Coni, G. Pichiri-Coni, G.M. Ledda-Columbano, P.M. Rao 1, S. Rajalakshmi 1, D.S.R. Sarma 1 and A. Columbano

Instituto di Farmacologia e Patologia Biochimica, Università di Cagliari Porcell 4, 09124 Cagliari, Italy
1Department of Pathology, Medical Sciences Building, University of Toronto Toronto, Ontario M5S 1A8, Canada

Experiments were designed to investigate the expression of three cell-cycle-dependent proto-oncogenes in response to two different types of proliferative stimuli: compensatory cell proliferation after partial hepatectomy (PH) or CCl4 and liver hyperplasia induced by the mitogens ethylene dibromide (EDB) and cyproterone acetate (CPA). Steady-state levels of messenger RNAs for c-fos and c-myc were found to be elevated after PH or CCI4 with a maximum increase between 0.5 and 2 h for c-fos and at 2–3 h for c-myc and a rapid decline after 3 h. However, when liver cell proliferation was induced by mitogens, no increase in the expression of c-fos mRNA was observed with both EDB or CPA during the first 24 h. In addition, elevated expression of c-myc was found only in liver hyperplasia induced by EDB, but not with CPA. While the expression of c-myc mRNA and c-fos mRNA was different in the two types of proliferative stimuli, that of c-Ha-ras and c-Ki-ras was similar in all the experimental groups. Cell proliferation monitored by means of incorporation of labelled thymidine into DNA or mitotic index at 24 h following PH, EDB and CPA occurred at a similar extent in all the experimental groups. Our data indicate that the transient and sequential expression of cell-cycle-related genes may vary in response to proliferative stimuli of different nature and suggest that increased expression of cell-cycle-related genes may not be a necessary prerequisite for the entry of the cells into the cell cycle


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