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© 1990 Oxford University Press

other

Radioresistant derivatives of an X-ray-senstive CHO cell line exhibit distinct patterns of sensitivity to DNA-damaging agents

Deborah R. Jaffe, Daniel Haraf, Jeffrey L. Schwartz, Ralph R. Weichselbaum and Alan M. Diamond 1

Department of Radiation and Cellular Oncology, University of Chicago Medical Center 5842 S. Maryland Ave. Chicago, IL 60637, USA

1To whom all correspondence should be addressed

X-ray-resistant clones of the xrs-5 radiosensitive derivative of the CHO-K1 cell line were generated by transfecting cosmid library DNAs into the X-ray-sensitive cells. Transfectants were selected for both a dominant drug resistance marker present in the vector sequences and return to wild-type survival. Three cell lines were isolated which show X-ray survival characteristics similar to parental K1 cells. These revertant lines were examined for their cross-sensitivity to cis-diamminedichloroplatinum(II) (cisplatin) and bleomycin. Although these cell lines reverted with regard to X-ray sensitivity, they retained their sensitivity to cisplatin. Furthermore, changes in bleomycin and X-ray sensitivity did not correlate. There was a positive correlation between return to wild-type radiosensitivity and an increase In the rate of DNA double-strand break rejoining.


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