© 1990 Oxford University Press
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32P-Postlabelling analysis of DNA adducted with urinary mutagens from smokers of black tobacco
1International Agency for Research on Cancer, 150 cours Albert-Thomas 69372 Lyon Cedex 8, France
2National Center for Toxicological Research Jefferson, AR 72079, USA
3University of Turin Via Santena 7, 10126 Turin, Italy
4Present address: National Institute for Research on Cancer, Viale Benedetto XV, 10, 1-16132 Genoa, Italy
5To whom correspondence should be addressed
In order to characterize the tobacco-derived mutagens excreted in the urine of tobacco smokers, 32P-postlabelling techniques were used to examine DNA adducts formed from these mutagens with calf thymus DNA in the presence of a metabolic activation system (rat liver S9, Aroclor 1254-induced, with or without acetyl coenzyme A). Using either nuclease P1 or butanol extraction procedures, four-six and three spots, respectively, were reproducibly found on the autoradiograms in the case of the urine extract from two smokers of black tobacco. Using the urinary extract from a non-smoker, only three faint spots were detected after nuclease P1 enrichment. DNA adducts produced in smokers' urine were then compared with those formed by four N-hydroxyarylamines, N-hydroxy-2-amino-3,8-dimethyl-3H- imidazo[4,5-f]quinoxaline N-hydroxy-2-amlno-3-methyl-imidazo[4,5-f]quinoline N-hydroxy-2-naphthylamine and N-hydroxy-4-aminobiphenyl. Visual inspection revealed that none of the reference aromatic amines contributed to the adduct pattern produced by the urinary mutagen(s). However, primary aromatic ainines are mainly implicated as urinary mutagens because: (i) they produce fraineshift mutations in Salmonella typhimurium strains, (ii) they are easily extractable with blue cotton and (iii) their mutagen icity is abolished by a nitrite treatment procedure for deamination.
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