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© 1990 Oxford University Press

other

32P-Postlabelling of 2-hydroxyethylated, ethylated and methytated adducts of 2'-deoxyguanosine 3'-monophosphate

Pertti Koivisto and Kari Hemminki

Institute of Occupational Health Topeliuksenkatu 41 a A, SF-00250 Helsinki, Finland

Ethylene oxide, diethyl sulphate and dimethyl sulphate were used to synthesize the corresponding 7-alkylation products of 2'-deoxyguanosine 3'-monophosphate (dGMP). The purified adducts were used as substrates in the 32P-postlabelling reaction with T4 polynucleotide kinase. The kinetics of phosphorylation were studied with 7-(2-hydroxyethyl)- dGMP: most net product was formed by 15 min and only a small increase was seen until 4 h. When different concen trations of the adducts were tested, a complete phosphoryl ation was noted for 7-methyl-dGMP to the lowest tested amount of 1 fmol. The efficiencies of phosphorylation for 7-ethyl- and 7-hydroxyethyl-dGMP were 1.5 and 0.5% respectively. The proportions phosphorylated were uniform over the concentration range tested. The results demonstrate dramatic differences in the efficiency of phosphorylation between structural analogues, which is probably related to a decreased affinity of the substrate to the enzyme or to an interference in the transfer of the phosphate group on the active site of the enzyme.


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