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© 1990 Oxford University Press

research-article

Stimulation of DNA synthesis carcinogen-induced hepatocytes in vitro

U. Klose, D. Thierau, U. Veser and L. R. Schwarz 1

Ingolstädter Landstrasse 1, GSF-Institut für Toxikologie 8042 Neuherberg, FRG

1To whom correspondence should be addressed

Diploid hepatocytes induced by a combination of diethylnitrosamine and 2-acetylaminofluorene were isolated and separated from polyploid hepatocytes by centrifugal elutriation. The diploid and polyploid cell fractions were ~90% pure and contained between 1 and 1.5x107 cells. When kept in monolayer cultures both cell populations responded to the mitogemc effect of EGF and insulin. However, the percentage of labelled nuclei was higher in predominantly diploid compared to predominantly tetraploid hepatocyte cultures at all epidermal growth factor (EGF) concentrations used in this study. At 10 ng EGF/ml and 10 mU insulin/ml the labelling index was twice as high in the diplold liver cells. Further work is required to show the relevance of the stronger response of the diploid cell fraction to mitogensin the process of carcinogenesis.


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