Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Dai, W.-D.
Right arrow Articles by O'Connor, P.J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dai, W.-D.
Right arrow Articles by O'Connor, P.J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1991 Oxford University Press

other

DNA methylation in specific cells of rat liver by N-nitrosodimethylamine and N-nitrosomethylbenzylamine

W.-D. Dai 2, V. Lee 1, W. Chin 1, D.P. Cooper, M.C. Archer and P.J. O'Connor

Cancer Research Camaign Section of Carcinogenesis, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute Wilmslow Road, Manchester M20 9BX, UK
1Department of Medical Biophysics, University of Toronto, Ontario Cancer Institute 500 Sherbourne Street, Toronto M4X 1K9, Canada
2Present address: Department of Pharmacology and Medicine, Royal Hallampshire Hospital Sheffield S10 2JF, UK

Dose-response curves for the O6-methylation of guanine in the hepatic DNA of Wistar and Sprague-Dawley rats were determined after administration of N-nitrosomethylbenzyl-amine (NMBzA) or N-nitrosodimethylamine (NDMA). Similar results were obtained for both rat strains but methylation of hepatic DNA by NDMA was {small tilde}9-fold more efficient than with NMBzA when doses were compared on a molar basis. Comparison by immunohistochemical analysis of the distribution of nuclei containing O6-methylguanine within the liver lobules showed that both agents tended to alkylate cells close to the central veins at the lower doses. With increasing doses, the band width of alkylated cells around the central vein increased, spreading in the case of NDMA virtually into the portal zones, whereas with NMBzA the zone of alkylated nuclei reached little more than halfway from the central vein to the portal zone. These differences in the distribution of alkylated cells may explain the differing hepatic responses to these two nitrosamines.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Toxicol PatholHome page
P. J. O'Connor, F. C.R. Manning, A. T. Gordon, M. A. Billett, D. P. Cooper, R. H. Elder, and G. P. Margison
Thresholds for Genotoxic Carcinogens: DNA Repair: Kinetics and Thresholds
Toxicol Pathol, May 1, 2000; 28(3): 375 - 381.
[Abstract] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
M. H. Mostafa, S. A. Sheweita, and P. J. O'Connor
Relationship between Schistosomiasis and Bladder Cancer
Clin. Microbiol. Rev., January 1, 1999; 12(1): 97 - 111.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol PatholHome page
V. M. Lee, R. G. Cameron, and M. C. Archer
Zonal Location of Compensatory Hepatocyte Proliferation Following Chemically Induced Hepatotoxicity in Rats and Humans
Toxicol Pathol, September 1, 1998; 26(5): 621 - 627.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.