© 1991 Oxford University Press
other |
Expression of E-cadherin, P-cadherin and involucrin by normal and neoplastic keratinocytes in culture
Keratinocyte Laboratory, Imperial Cancer Research Fund PO Box 123, Lincoln's Inn Fields, London WC2A 3PX, UK
1Present address: Department of Pathology, Georgetown University Medical School 3900 Reservpor Road, NW, Washington DC 20007, USA
We have compared expression of involucrin, E-cadherin and P-cadherin in cultures of normal keratinocytes and in five different lines derived from squamous cell carcinomas (SCCs), using Northern analysis and immunofluorescence. In normal keratinocytes there was an inverse correlation between P-cadherin and involucrin expression, whereas E-cadherin was expressed by both basal and terminally differentiating cells. In SCC lines involucrin expression was lower than in normal keratinocytes, and there was variable expression of P- and E-cadherin: E-cadherin mRNA levels tended to be lower in SCC lines than in normal keratinocytes, whereas P-cadherin levels were similar. Our results are consistent with observations of cadherin expression in vivo and suggest that the cultures provide a useful experimental model for investigating the role of cadherins in determining the spatial organization of normal and neoplastic keratinocytes.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Levy, S. Broad, D. Diekmann, R. D. Evans, and F. M. Watt beta 1 Integrins Regulate Keratinocyte Adhesion and Differentiation by Distinct Mechanisms Mol. Biol. Cell, February 1, 2000; 11(2): 453 - 466. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. J. de Boer, E. van Dorst, H. van Krieken, C. M. Jansen-van Rhijn, S. O. Warnaar, G. J. Fleuren, and S. V. Litvinov Changing Roles of Cadherins and Catenins during Progression of Squamous Intraepithelial Lesions in the Uterine Cervix Am. J. Pathol., August 1, 1999; 155(2): 505 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z Li, W. Gallin, G Lauzon, and M Pasdar L-CAM expression induces fibroblast-epidermoid transition in squamous carcinoma cells and down-regulates the endogenous N-cadherin J. Cell Sci., January 4, 1998; 111(7): 1005 - 1019. [Abstract] [PDF]
|
|
|
|
|
|
A. Zhu and F. Watt Expression of a dominant negative cadherin mutant inhibits proliferation and stimulates terminal differentiation of human epidermal keratinocytes J. Cell Sci., January 12, 1996; 109(13): 3013 - 3023. [Abstract] [PDF]
|
|
|
|
|
|
M Rabino, L Trusolino, M Prat, O Cremona, P Savoia, and P. Marchisio A monoclonal antibody identifies a novel GPI-anchored glycoprotein involved in epithelial intercellular adhesion J. Cell Sci., January 6, 1994; 107(6): 1413 - 1428. [Abstract] [PDF]
|
|
|
|
|
|
A Tang, M. Eller, M Hara, M Yaar, S Hirohashi, and B. Gilchrest E-cadherin is the major mediator of human melanocyte adhesion to keratinocytes in vitro J. Cell Sci., January 4, 1994; 107(4): 983 - 992. [Abstract] [PDF]
|
|