© 1991 Oxford University Press
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Endogenous N-nitrosation in man assessed by measurement of apparent total N-nitroso compounds in faeces
BIBRA Woodmansterne Road, Carshalton, Surrey SM5 4DS, UK
1Norsk Hydro Research Center 3901 Porsgrunn, Norway
2Ministry of Agriculture, Fisheries and Food, Food Safety Directorate, Food Science Laboratory Colney Lane, Norwich NR4 7UG, UK
The faecal concentration of substances responding to the chemical test for N-nitroso compounds (apparent total N-nitroso compounds, ATNC) was investigated in human subjects consuming their normal free-choice diet. Concentrations ranged from 40 to 590 µg (N-NO)/kg faeces. To ascertain the likely relative contributions of endogenous ATNC formation and preformed, dietary ATNC, the subjects consumed a diet low in nitrate and ATNC for 8 days. At the end of this period, ATNC had decreased substantially with concentrations ranging from below the 40 µg (N-NO)/kg detection limit up to 143 µg (N-NO)/kg, mean 82 µg (N-NO)/kg. On supplementing this diet with 300 mg nitrate/day, faecal ATNC levels increased markedly. On the third day of this regime, values were in the range 73–714 µg (N-NO)/kg with a mean of 307 µg (N-NO)/kg. The results, together with the known limited occurrence of ATNC in the majority of foodstuffs so far tested, generally non-detectable or <100 µg (N-NO)/kg, suggest that endogenous formation via species derived from dietary nitrate is likely to be an important source of ATNC in human faeces.
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