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© 1991 Oxford University Press

research-article

32P-Postlabelling analysis and micronuclei induction in primary Chinese hamster lung cells exposed to tobacco particulate matter

Nigel J. Jones, Munira A. Kadhim 1, Peter L. Hoskins, James M. Parry and Raymond Waters

Molecular Biology Research Group, School of Biological Sciences, University College of Swansea Singleton Park, Swansea SA2 8PP, UK

The genotoxicity of tobacco particulate matter (TPM) derived from a low-tar, low-nicotine cigarette has been examined by measuring micronucleus induction in a primary pulmonary cell line, both in the absence and presence of an exogenous source of metabolic activation. In an attempt to correlate the cytogenetic damage observed with DNA adduct formation, DNA extracted from TPM-treated cells has been analysed with two different modifications of the 32P-postlabethng assay. The results from the 32P-postlabelling analysis taken together with the pattern of micronucleus induction provide strong evidence that bioreactivated aromatic carcinogens, such as benzo[a]pyrene, are unlikely to be responsible for the TPM-induced cytogenetic damage observed in cultured mammalian cells.


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