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© 1991 Oxford University Press

research-article

Alteration in restriction enzyme digestion patterns detects DNA-protein complexes induced by chromate

Yong Chen, Mitchell D. Cohen, E.T. Snow and Max Costa 1

Institute of Environmental Medicine, New York University Medical Center 550 First Avenue, New York, NY 10016, USA

1To whom correspondence should be addressed

DNA-protein complexes isolated from CHO cells treated with at least 10–30 µM potassaium chromate exhibited an alteration in the degradation of the DNA by restriction enzymes compared to DNA-protein complexes isolated from untreated cells. This alteration in restriction enzymes digestion of DNA-protein complexes induced by chromate was shown to depend upon the b inding of trivalent chromium to the DNA and upon the protein associated with the DNA, since both EDTA pretreatment and protease K reversed the inhibition of restriction enzyme degradation of the DNA. The inhibition of restriction enzyme degradation of DNA-protein complexes may be utilized as an indirect way to detect DNA-protein complexing induced by chromate and perhaps other agents.


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