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© 1991 Oxford University Press

research-article

Immortalization of normal human oral keratinocytes with type 16 human papillomavirus

No-Hee Park 1 2 4, Byung-Moo Min 1, Sheng-lin Li 1, Min Zhong Huang 1 and Jay Doniger 3

1UCLA School of Dentistry Los Angeles, CA 90024, USA
2UCLA Jonsson Comprehensive Cancer Center Los Angeles, CA 90024, USA
3Georgetown University School of Medicine Washington, DC 20007, USA

4To whom correspondence should be sent at the Section of Oral Biology, UCLA School of Dentistry, 43433 CHS, 10833 Le Conte Avenue, Los Angeles, CA 90024, USA

Primary human oral keratinocytes were transformed by transfection with recombinant human papillomavirus type 16 (HPV-16)DNA, and two transformed cell lines named human oral keratinocytes-16A and -16B (HOK-16A and HOK-16B) were established. While normal cells and cells transfected with vector only exhibited a limited Wespan, the HOK-16A and HOK-16B lines demonstrated immortality and altered morphology from their normal counterpart. The HOK-16A and HOK-16B lines contained {small tilde}40 and {small tilde}25 copies of intact HPV-16 DNA as integrated form per cell respectively, and both cell lines exprpssed several viral specific poly(A+) RNAs. Notably these cell lines also overexpressed cellular myc proto-oncogene in comparison with the normal counterpart. However, the immortalized cell lines were not able to produce tumors in nude mice, indicating that the cells are partially transformed. The HOK-16A and HOK-16B lines are, therefore, useful for investigating the multistep molecular events of oral carcinogenesis.


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