Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (32)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Spigelman, A. D.
Right arrow Articles by Phillips, R. K.S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Spigelman, A. D.
Right arrow Articles by Phillips, R. K.S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1991 Oxford University Press

research-article

DNA adducts, detected by 32P-postlabelling, in the foregut of patients with familial adenomatous polyposis and in unaffected controls

Allan D. Spigelman 1 2, Debra K. Scates 3, Stanley Venitt 3 4 and Robin K.S. Phillips 1

1St Mark's Hospital London
2Academic Surgical Unit, St Mary's Hospital London
3Cancer Research Campaign Section of Molecular Carcinogenesis, Haddow Laboratories, Institute of Cancer Research Royal Marsden Hospital, Sutton, Surrey, SM2 5NG, UK

4To whom correspondence should be addressed

Duodenal neoplasms in patients with familial adenomatous polyposis (FAP) cluster around the ampulla; duodenal neoplasia is common but gastric neoplasia is rare. These observations suggest that bile enhances neoplasia by carrying carcinogens to target cells. A critical step in carcinogenesis is the reaction of carcinogens with DNA to form chemical adducts. Using 32P-postlabelling to measure DNA adducts we asked: (i) are there more adducts in the duodenum of patients with FAP than in the duodenum of normal patients? (ii) Are there more adducts in the duodenum than in the stomach? We measured adducts in duodenal biopsies from 51 patients with FAP and 30 age-matched controls; and paired gastric and duodenal biopsies from 31 FAP patients and six controls. The foregut of 90% of all patients studied contained DNA modifications with characteristics of aromatic non-polar DNA adducts. In duodenal biopsies, adduct labelling per 109 DNA nucleotides was significantly higher in FAP patients (median 15.0, range 0–162) than in normal patients (median, 7.5, range 0–40; P = 0.0002). In paired duodenal and gastric biopsies from 31 FAP patients, adduct labelling was significantly higher in duodenal DNA (median 15, range 0–109) than in stomach DNA (median 8.0, range 0–40; P = 0.0004). Age, gender or smoking did not account for these differences. Gastric adducts (median 3.5, range 0–10) were lower than duodenal adducts (median 8.5, range 0–29) in paired biopsies from six control patients, P = 0.031). These results support the hypothesis that pancreaticobiliary secretions may be involved in the pathogenesis of foregut neoplasia in FAP.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GutHome page
S A Khan, P L Carmichael, S D Taylor-Robinson, N Habib, and H C Thomas
DNA adducts, detected by 32P postlabelling, in human cholangiocarcinoma
Gut, April 1, 2003; 52(4): 586 - 591.
[Abstract] [Full Text] [PDF]

Home page
 Arch SurgHome page
A. D. Spigelman, S. Venitt, and R. K. S. Phillips
Bile and the Growth of Intestinal Polyps
Arch Surg, March 1, 1992; 127(3): 361 - 361.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.