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© 1992 Oxford University Press

research-article

Chromosomal, mitochondrial and metabolic alterations in SV40-transformed rabbit chondrocytes

A. Bravard 1, J. Beaumatin 1, C. Luccioni 1, P. Fritsch 2, D. Lefrançois 3, S. Thenet 4, M. Adolphe 4 and B. Dutrillaux 1 3

1 CEA/DSV/DPTE/LCG, BP no. 6 92265 Fontenay-aux-roses Cedex
2 CEA/DSV/DPTE/STCE, BP no. 6 92265 Fontenay-aux-roses Cedex
3 URA 620, CNRS, Institut Curie, Section Biologie, 26 rue d'Ulm, 75231 Paris Cedex 05
4Laboratoire de Pharmacologie Cellulaire de I'EPHE, Institut Biomedical des Cordeliers 15 rue de l'Ecole de Medecine, 75006 Paris, France

Karyotype, mitochondrial ultrastructure and several enzymatic activities were studied in two clones, D22 and D27, from SV40-transformed rabbit chondrocytes. Similar chromosome alterations, with recurrent losses and gains were observed at thevarious passages. Mitochondria were rare, with increase in size and crest alterations. By comparison to non-transformed rabbit chondrocytes, activities of superoxide dismutase 1 and 2 (SOD) and glutathione peroxidase were increased, those of glutathione reductase, glucose-6-phosphate dehydrogenase and glutathione-S-transferase fluctuated according to passages, thymidylate synthase decreased, thymidine kinase and hypoxanthine-phosphoribosyl-trans-ferase increased and the ratio lactatedehydrogenase B/A increased. In most cases, these variations were correlated with the number of chromosomes carrying the genes encoding for corresponding enzymes. These results, compared to those obtained in SV40-transformed human fibroblasts, demonstrate that the two cell types behave differently for detoxica-tion systems against oxygen radicals, in particular for SOD2 activity, and have opposite imbalances of chromosomes carrying the corresponding genes.


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