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© 1992 Oxford University Press

other

Tumorigenic activity of a rearranged c-myc gene from a human T-cell leukemia line

Daniela Petroni 1, Paola Comi 1 2, Barbara Giglioni 2, Alessandra Stacchini 3, Giovanna Martinotti 3, Angelo Guerrasio 4 and Giuseppe Saglio 4

1Dipartimento di Scienze e Tecnologie Biomediche, Universita' di Milano Milano
2Centro di Studio sulla Patologia Cellulare-CNR Milano
3Istituto di Microbiologia. Universita' di Torino Italy
4Dipartimento di Scienze Biomediche e Oncologia Umana, Universita' di Torino Italy

The T-lymphoma cell line Hut78 contains a rearranged c-myc oncogene derived from a translocation between the long arms of chromosomes 8 and 2; the event deletes the 3' end of the gene, causing the loss of the transcribed AT-rich sequence. It has recently been shown that the mutant c-myc mRNA is several-fold more stable than normal c-myc mRNA. We have assessed the tumorigenicity of the mutant c-myc allele by transfecting this gene and its normal counterpart into NIH3T3 cells, together with a neomycin resistance gene. Following selection for G-418 resistance, the cells were injected into nude mice. Tumors containing integrated c-myc arose in animals injected with cells transfected by the mutated, but not by the normal, allele. The results suggest that this rearranged c-myc bears a tumorigenic activity not observed in other naturally occurring mutated c-myc alleles and may have directly contributed to the twuorigenic event in the Hut78 cell line.


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