© 1992 Oxford University Press
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Mutagenesis by (+)-anti-B[
]P-N2-Gua, the major adduct of activated benzo[]pyrene, when studied in an Escherichia coli plasmid using site-directed methods
Department of Biology, Boston University Boston, MA 02215, USA
1To whom reprint requests should be sent
The suspected major mutagenic adduct of benzo[
]pyrene, (+)-anti-B[]P-N2-Gua, is built into the unique PstI recognition site of the Escherichia coli plasmid, pUC19, in order to study its mutagenic potential. The adduct can either be at G437, which is replicated during leading strand DNA synthesis, or at G438, which is replicated during lagging strand DNA synthesis. The DNA strand complementary to the strand containing the (+)-anti-B[]P-N2-Gua adduct is saturated with UV lesions to minimize its potential to generate progeny. Although all in-frame mutations could have been detected, a G437 
T transversion mutation is virtually exclusively obtained at a frequency of {small tilde}0.04% per adduct following transformation into Uvr+ E.coli when SOS is not induced, and {small tilde}0.18% when SOS is induced. The mutation frequency of the adduct in a Uvr– backgraund is estimated to be {small tilde}0.2% when SOS is not induced, and {small tilde}0.9% when SOS is induced. The absence of G438 T mutations is rationalized. G T mutations from (+)-anti-B[]P-N2-Gua are compared to the mutational specificity of the ultimate mutagenic form of activated benzo[]pyrene.
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