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© 1993 Oxford University Press

research-article

Biochemical and histochemical properties of hepatic tumors of rainbow trout, Oncorhynchus mykiss

Lynn M. Parker, Darrel J. Laurén 1, Bruce D. Hammock 2, Bruce Winder 2 and David E. Hinton

Department of Medicine, School of Veterinary Medicine, University of California Davis, CA 95616
1EA Engineering, Science and Technology 41 Lafayette Circle, Lafayette, CA 94549
2Department of Entomology and Environmental Toxicology, University of California Davis, CA 95616, USA

Biochemical and histochemical studies were conducted in aflatoxin B1-induced liver tumors in adult, rainbow trout. Specific activities of the phase I enzymes, ethoxyresorufin-O-deethylase (EROD), microsomal and cytosolic epoxide hydrolase (mEH and cEH), aldehyde dehydrogenase (ALDH) and DT-diaphorase, and the phase II enzymes, {gamma}-glutamyltransferase ({gamma}-GT), glutathione transferase (GST) and uridine diphosphoglucuronyl transferase (UDPGT) were measured. Cryostat sections of tumor and surrounding liver from the same cohorts were analyzed immunohistochemically for cytochrome P450IA1 and histochemically for ALDH (benzaldehyde and hexanal), DT-diaphorase, {gamma}-GT and uridine diphosphoglucuronyl dehydrogenase (UDPGdH). In tumor tissues, the largest biochemical changes were found with benzaldehyde dehydrogenase, where activity increased from undetectable levels to 7.4 nmol/min/mg protein, and {gamma}-GT, where activity increased 12-fold over controls. Increases in other enzymes ranged from 1.26 to 2.84 times that of control liver, except EROD, which decreased, and cEH and mEH, which were unchanged. Histochemical analyses showed mEH, which were unchanged. Hischemical analyses showed the induction of ALDH, {gamma}-GT, DT-diaphorase and UDPGdH, and the depression of cytochrome P450IA1 in hepatic neoplasms. In addition, marker enzyme histochemistry of neoplasms revealed heterogeneous populations of hepatocytes and absence of necrotic areas.


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 Toxicol PatholHome page
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