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© 1993 Oxford University Press

research-article

Production of scatter factor-like activity by a nitrosamine-induced pancreatic cancer cell line

Masahiko Hirota 1, Hiroshi Egami 1, Stefano Corra 1, Hideki Fujii 1, William G. Chaney 1 2, Angie Rizzino 1 and Parviz M. Pour 1 3

1The Eppley Institute for Research in Cancer and Allied Diseases Omaha, NE 68198-6805, USA
2Department of Biochemistry, University of Nebraska Medical Center Omaha, NE 68198–6805, USA

3To whom correspondence should be addressed

Two hamster pancreatic cancer cell lines, PC-1 and PC1.0, established from N-nitrosobis(2-oxopropyl)amine-induced pancreatic ductal/ductular adenocarcinomas exhibit different growth patterns. PC-1 cells, which produce well differentiated adenocarcinomas in vitro after allogeneic inoculation, form cell aggregates and characteristic island-like structures in vitro. PC1.0 cells, which produce poorly differentiated tumors in vivo, form dispersed colonies in vitro. Conditioned medium prepared from PC1.0 cells inhibits PC-1 cells from forming island-like colonies. The conditioned medium also prevents several human pancreatic carcinoma cell lines, HPAF, CD11 and CD18, from forming compact colonies. These properties are similar to those described previously as scatter factors. The scatter factor-like activity is heat-labile, acid-stable, non-dialyzable, trypsin sensitive and unaffected by reducing agents. The activity is not suppressed by addition of heparin, and it does not bind to heparin. In addition, the scatter phenomenon is not reproduced by acidic or basic fibroblast growth factor, epidermal growth factor or transforming growth factor-ß1. Based on these findings, it appears that the scattering activity produced by PC1.0 cells differs from the scatter factors that have been identified in other systems.


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