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© 1993 Oxford University Press

research-article

Decrease in catalase activity and loss of the 11p chromosome arm in the course of SV40 transformation of human fibroblasts

F. Hoffschir 1, M. Vuillaume 2, L. Sabatier 1, M. Ricoul 1, L. Daya-Grosjean 3, S. Estrade 3, R. Cassingena 3, R. Calvayrac 4, A. Sarasin 3 and B. Dutrillaux 1 5

1CEA/DSV/DPTE/LCG, BP6 92265 Fontenay-aux-Roses
2URA 620 CNRS, Ecole Normale Supérieure 46 rue d'Ulm, 75230 Paris
3UPR 42 CNRS, IRSC BP8 94801 Villejuif
4Laboratoirc des Membranes Biologiques, Université Paris VII 2 place Jussieu, 75005 Paris
5URA 620 CNRS, Institut Curie, 26 rue d'Ulm 75231 Paris Cedex 05, France

The activity of catalase, a key enzyme in cell detoxication of oxygen derivatives, was studied in SV40 transformed human fibroblasts. A cytogenetic study was performed in parallel to establish a quantification of 11p arm on which the corresponding gene is mapped. mRNA amounts were determined by Northern blotting. At early passages, catalase activity strongly decreased whereas the corresponding mRNA was present. No deletions of lip arms were detected. At later passages, catalase activity remained low. lip arm deletions were frequent, and the amount of mRNA was decreased. In these late passages, the good correlation between the number of lip arms and catalase activity suggested a gene dosage effect. It is assumed that the decrease of catalase activity provides a selective advantage for the transformed cells. This decrease is related to a post-transcriptional change of regulation at early passages and to the loss of the corresponding gene at later passages.


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