© 1993 Oxford University Press
other |
Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA
Rowett Research Institute Greenburn Road, Bucksburn, Aberdeen AB2 9SB, UK
The endogenous production of oxidative damage in DNA by free radicals released as a by-product of respiration is a likely cause of mutations which, if they occur in appropriate genes, may lead to cancer. Using an endonuclease specific for oxidized pyrimidines, in conjunction with the highly sensitive method of single cell gel electrophoresis, we have detected significant oxidative damage in untreated, freshly isolated lymphocytes from normal, healthy individuals.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Rybanska, J. Gursky, M. Faskova, E. P. Salazar, E. Kimlickova-Polakovicova, K. Kleibl, L. H. Thompson, and M. Pirsel Newly identified CHO ERCC3/XPB mutations and phenotype characterization Mutagenesis, December 11, 2009; (2009) gep059v2. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Azqueta, Y. Lorenzo, and A. R. Collins In vitro comet assay for DNA repair: a warning concerning application to cultured cells Mutagenesis, July 1, 2009; 24(4): 379 - 381. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Gajski and V. Garaj-Vrhovac Radioprotective Effects of Honeybee Venom (Apis mellifera) Against 915-MHz Microwave Radiation-Induced DNA Damage in Wistar Rat Lymphocytes: In Vitro Study International Journal of Toxicology, March 1, 2009; 28(2): 88 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Serpeloni, M. Bisarro dos Reis, J. Rodrigues, L. Campaner dos Santos, W. Vilegas, E. A. Varanda, A. L. Dokkedal, and I. M. S. Colus In vivo assessment of DNA damage and protective effects of extracts from Miconia species using the comet assay and micronucleus test Mutagenesis, November 1, 2008; 23(6): 501 - 507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Staruchova, A. R. Collins, K. Volkovova, C. Mislanova, Z. Kovacikova, J. Tulinska, A. Kocan, L. Staruch, L. Wsolova, and M. Dusinska Occupational exposure to mineral fibres. Biomarkers of oxidative damage and antioxidant defence and associations with DNA damage and repair Mutagenesis, July 1, 2008; 23(4): 249 - 260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Kaymak, E Kadioglu, E Ozcagli, G Osmanoglu, S Izdes, C Agalar, H Basar, and S Sardas Oxidative DNA damage and total antioxidant status in rats during experimental gram-negative sepsis Human and Experimental Toxicology, June 1, 2008; 27(6): 485 - 491. [Abstract] [PDF]
|
|
|
|
|
|
A. R. Collins, A. A. Oscoz, G. Brunborg, I. Gaivao, L. Giovannelli, M. Kruszewski, C. C. Smith, and R. Stetina The comet assay: topical issues Mutagenesis, May 1, 2008; 23(3): 143 - 151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dusinska and A. R. Collins The comet assay in human biomonitoring: gene-environment interactions Mutagenesis, May 1, 2008; 23(3): 191 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Romieu, F. Castro-Giner, N. Kunzli, and J. Sunyer Air pollution, oxidative stress and dietary supplementation: a review Eur. Respir. J., January 1, 2008; 31(1): 179 - 197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Li, X. Jia, C.-Y. O. Chen, J. B. Blumberg, Y. Song, W. Zhang, X. Zhang, G. Ma, and J. Chen Almond Consumption Reduces Oxidative DNA Damage and Lipid Peroxidation in Male Smokers J. Nutr., December 1, 2007; 137(12): 2717 - 2722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Witte, U. Plappert, H. de Wall, and A. Hartmann Genetic Toxicity Assessment: Employing the Best Science for Human Safety Evaluation Part III: The Comet Assay as an Alternative to In Vitro Clastogenicity Tests for Early Drug Candidate Selection Toxicol. Sci., May 1, 2007; 97(1): 21 - 26. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. I. Gill, S. Haldar, L. A Boyd, R. Bennett, J. Whiteford, M. Butler, J. R Pearson, I. Bradbury, and I. R Rowland Watercress supplementation in diet reduces lymphocyte DNA damage and alters blood antioxidant status in healthy adults Am. J. Clinical Nutrition, February 1, 2007; 85(2): 504 - 510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Arbillaga, A. Azqueta, O. Ezpeleta, and A. L. d. Cerain Oxidative DNA damage induced by Ochratoxin A in the HK-2 human kidney cell line: evidence of the relationship with cytotoxicity Mutagenesis, January 1, 2007; 22(1): 35 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. R. Ferguson, M. Philpott, and N. Karunasinghe Oxidative DNA Damage and Repair: Significance and Biomarkers J. Nutr., October 1, 2006; 136(10): 2687S - 2689S. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Koptyra, R. Falinski, M. O. Nowicki, T. Stoklosa, I. Majsterek, M. Nieborowska-Skorska, J. Blasiak, and T. Skorski BCR/ABL kinase induces self-mutagenesis via reactive oxygen species to encode imatinib resistance Blood, July 1, 2006; 108(1): 319 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Smith, M. R. O'Donovan, and E. A. Martin hOGG1 recognizes oxidative damage using the comet assay with greater specificity than FPG or ENDOIII Mutagenesis, May 1, 2006; 21(3): 185 - 190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mocali, L. Giovannelli, P. Dolara, and F. Paoletti The Comet Assay Approach to Senescent Human Diploid Fibroblasts Identifies Different Phenotypes and Clarifies Relationships Among Nuclear Size, DNA Content, and DNA Damage J. Gerontol. A Biol. Sci. Med. Sci., June 1, 2005; 60(6): 695 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Ziel, V. Grishko, C. C. Campbell, J. F. Breit, G. L. Wilson, and M. N. Gillespie Oxidants in signal transduction: impact on DNA integrity and gene expression FASEB J, March 1, 2005; 19(3): 387 - 394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Lee, N. J. Hodges, and J. K. Chipman Interindividual Variability in Response to Sodium Dichromate-Induced Oxidative DNA Damage: Role of the Ser326Cys Polymorphism in the DNA-Repair Protein of 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine DNA Glycosylase 1 Cancer Epidemiol. Biomarkers Prev., February 1, 2005; 14(2): 497 - 505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Migliore, S. Molinu, A. Naccarati, M. Mancuso, A. Rocchi, and G. Siciliano Evaluation of cytogenetic and DNA damage in mitochondrial disease patients: effects of coenzyme Q10 therapy Mutagenesis, January 1, 2004; 19(1): 43 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Moller, U. Vogel, A. Pedersen, L. O. Dragsted, B. Sandstrom, and S. Loft No Effect of 600 Grams Fruit and Vegetables Per Day on Oxidative DNA Damage and Repair in Healthy Nonsmokers Cancer Epidemiol. Biomarkers Prev., October 1, 2003; 12(10): 1016 - 1022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Alleva, M. Tomasetti, M. D. Solenghi, F. Stagni, F. Gamberini, A. Bassi, P. M. Fornasari, G. Fanelli, and B. Borghi Lymphocyte DNA damage precedes DNA repair or cell death after orthopaedic surgery under general anaesthesia Mutagenesis, September 1, 2003; 18(5): 423 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Sparrow, J. Zhou, and B. Cai DNA Is a Target of the Photodynamic Effects Elicited in A2E-Laden RPE by Blue-Light Illumination Invest. Ophthalmol. Vis. Sci., May 1, 2003; 44(5): 2245 - 2251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. M. R. Broekmans, I. A. A. Klopping-Ketelaars, J. A. Weststrate, L. B. M. Tijburg, G. van Poppel, A. A. Vink, T. T.J.M Berendschot, M. L. Bots, W. A. M. Castenmiller, and A. F. M. Kardinaal Decreased Carotenoid Concentrations Due to Dietary Sucrose Polyesters Do Not Affect Possible Markers of Disease Risk in Humans J. Nutr., March 1, 2003; 133(3): 720 - 726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. T. Mayne Antioxidant Nutrients and Chronic Disease: Use of Biomarkers of Exposure and Oxidative Stress Status in Epidemiologic Research J. Nutr., March 1, 2003; 133(3): 933S - 940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hartmann, E. Agurell, C. Beevers, S. Brendler-Schwaab, B. Burlinson, P. Clay, A. Collins, A. Smith, G. Speit, V. Thybaud, et al. Recommendations for conducting the in vivo alkaline Comet assay Mutagenesis, January 1, 2003; 18(1): 45 - 51. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Ravanat, T. Douki, P. Duez, E. Gremaud, K. Herbert, T. Hofer, L. Lasserre, C. Saint-Pierre, A. Favier, and J. Cadet Cellular background level of 8-oxo-7,8-dihydro-2'-deoxyguanosine: an isotope based method to evaluate artefactual oxidation of DNA during its extraction and subsequent work-up Carcinogenesis, November 1, 2002; 23(11): 1911 - 1918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Andreassi, N. Botto, A. Rizza, M. G. Colombo, C. Palmieri, S. Berti, S. Manfredi, S. Masetti, A. Clerico, and A. Biagini Deoxyribonucleic acid damage in human lymphocytes after percutaneous transluminal coronary angioplasty J. Am. Coll. Cardiol., September 4, 2002; 40(5): 862 - 868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Giovannelli, C. Saieva, G. Masala, G. Testa, S. Salvini, V. Pitozzi, E. Riboli, P. Dolara, and D. Palli Nutritional and lifestyle determinants of DNA oxidative damage: a study in a Mediterranean population Carcinogenesis, September 1, 2002; 23(9): 1483 - 1489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Moller and S. Loft Oxidative DNA damage in human white blood cells in dietary antioxidant intervention studies Am. J. Clinical Nutrition, August 1, 2002; 76(2): 303 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Migliore, L. Petrozzi, C. Lucetti, G. Gambaccini, S. Bernardini, R. Scarpato, F. Trippi, R. Barale, G. Frenzilli, V. Rodilla, et al. Oxidative damage and cytogenetic analysis in leukocytes of Parkinson's disease patients Neurology, June 25, 2002; 58(12): 1809 - 1815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.J. Duthie, L. Pirie, A.McE. Jenkinson, and S. Narayanan Cryopreserved versus freshly isolated lymphocytes in human biomonitoring: endogenous and induced DNA damage, antioxidant status and repair capability Mutagenesis, May 1, 2002; 17(3): 211 - 214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Potter, K. A. Gollahon, B. J.A. Palanca, M. J. Harbert, Y. M. Choi, A. H. Moskovitz, J. D. Potter, and P. S. Rabinovitch Flow cytometric analysis of the cell cycle phase specificity of DNA damage induced by radiation, hydrogen peroxide and doxorubicin Carcinogenesis, March 1, 2002; 23(3): 389 - 401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, S.-Y. Chen, T. Hsu, and R. M. Santella Immunohistochemical detection of malondialdehyde-DNA adducts in human oral mucosa cells Carcinogenesis, January 1, 2002; 23(1): 207 - 211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Narayanan, M.R. O'Donovan, and S.J. Duthie Lysis of whole blood in vitro causes DNA strand breaks in human lymphocytes Mutagenesis, November 1, 2001; 16(6): 455 - 459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N.J. Hodges, B. Adam, A.J. Lee, H.J. Cross, and J.K. Chipman Induction of DNA-strand breaks in human peripheral blood lymphocytes and A549 lung cells by sodium dichromate: association with 8-oxo-2-deoxyguanosine formation and inter-individual variability Mutagenesis, November 1, 2001; 16(6): 467 - 474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Benitez-Bribiesca, P. Sanchez, J. Toledo, R. Penarroja, M. Flores, and J. Sosa Differential Staining of DNA Strand Breaks in Dried Comet Assay Slides J. Histochem. Cytochem., July 1, 2001; 49(7): 921 - 922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. van den Berg, T. van Vliet, W. M. R. Broekmans, N. H. P. Cnubben, W. H. J. Vaes, L. Roza, G. R.M.M. Haenen, A. Bast, and H. van den Berg A Vegetable/Fruit Concentrate with High Antioxidant Capacity Has No Effect on Biomarkers of Antioxidant Status in Male Smokers J. Nutr., June 1, 2001; 131(6): 1714 - 1722. [Abstract] [Full Text]
|
|
|
|
|
|
A.B. Nia, F.J. Van Schooten, P.A.E.L. Schilderman, T.M.C.M. De Kok, G.R. Haenen, M.H.M. Van Herwijnen, E. Van Agen, D. Pachen, and J.C.S. Kleinjans A multi-biomarker approach to study the effects of smoking on oxidative DNA damage and repair and antioxidative defense mechanisms Carcinogenesis, March 1, 2001; 22(3): 395 - 401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Møller, L. E. Knudsen, S. Loft, and H. Wallin The Comet Assay as a Rapid Test in Biomonitoring Occupational Exposure to DNA-damaging Agents and Effect of Confounding Factors Cancer Epidemiol. Biomarkers Prev., October 1, 2000; 9(10): 1005 - 1015. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Ip, H. J. Thompson, Z. Zhu, and H. E. Ganther In Vitro and in Vivo Studies of Methylseleninic Acid: Evidence That a Monomethylated Selenium Metabolite Is Critical for Cancer Chemoprevention Cancer Res., June 1, 2000; 60(11): 2882 - 2886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.D. BRUNER, D.P.G. NORMAN, J.C. FROMME, and G.L. VERDINE Structural and Mechanistic Studies on Repair of 8-Oxoguanine in Mammalian Cells Cold Spring Harb Symp Quant Biol, January 1, 2000; 65(0): 103 - 112. [Abstract] [PDF]
|
|
|
|
|
|
A. P. McGlynn, G. Wasson, J. O'Connor, V. J. McKelvey-Martin, and C. S. Downes The Bromodeoxyuridine Comet Assay: Detection of Maturation of Recently Replicated DNA in Individual Cells Cancer Res., December 1, 1999; 59(23): 5912 - 5916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Rieger, A. Parlesak, B. L. Pool-Zobel, G. Rechkemmer, and C. Bode A diet high in fat and meat but low in dietary fibre increases the genotoxic potential of `faecal water' Carcinogenesis, December 1, 1999; 20(12): 2311 - 2316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fenech, J. Crott, J. Turner, and S. Brown Necrosis, apoptosis, cytostasis and DNA damage in human lymphocytes measured simultaneously within the cytokinesis-block micronucleus assay: description of the method and results for hydrogen peroxide Mutagenesis, November 1, 1999; 14(6): 605 - 612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Andreoli, P. Leopardi, S. Rossi, and R. Crebelli Processing of DNA damage induced by hydrogen peroxide and methyl methanesulfonate in human lymphocytes: analysis by alkaline single cell gel electrophoresis and cytogenetic methods Mutagenesis, September 1, 1999; 14(5): 497 - 504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Pool-Zobel, S. L. Abrahamse, A. R. Collins, W. Kark, R. Gugler, D. Oberreuther, E. G. Siegel, S. T.-v. Lishaut, and G. Rechkemmer Analysis of DNA Strand Breaks, Oxidized Bases, and Glutathione S-Transferase P1 in Human Colon Cells from Biopsies Cancer Epidemiol. Biomarkers Prev., July 1, 1999; 8(7): 609 - 614. [Abstract] [Full Text]
|
|
|
|
|
|
R. S. Tebbs, J. E. Cleaver, R. A. Pedersen, and A. Hartmann Modification of the Comet assay for the detection of DNA strand breaks in extremely small tissue samples Mutagenesis, July 1, 1999; 14(4): 437 - 438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Prise, C. H.L. Pullar, and B. D. Michael A study of endonuclease III-sensitive sites in irradiated DNA: detection of {alpha}-particle-induced oxidative damage Carcinogenesis, May 1, 1999; 20(5): 905 - 909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Will, H.-C. Mahler, A.-P. Arrigo, and B. Epe Influence of glutathione levels and heat-shock on the steady-state levels of oxidative DNA base modifications in mammalian cells Carcinogenesis, February 1, 1999; 20(2): 333 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Duthie and A. Hawdon DNA instability (strand breakage, uracil misincorporation, and defective repair) is increased by folic acid depletion in human lymphocytes in vitro FASEB J, November 1, 1998; 12(14): 1491 - 1497. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. L. Croteau and V. A. Bohr Repair of Oxidative Damage to Nuclear and Mitochondrial DNA in Mammalian Cells J. Biol. Chem., October 10, 1997; 272(41): 25409 - 25412. [Full Text] [PDF]
|
|
|
|
 |
|
 R. Brooks and D. Winton Determination of spatial patterns of DNA damage and repair in intestinal crypts by multi-cell gel electrophoresis J. Cell Sci., January 8, 1996; 109(8): 2061 - 2068. [Abstract] [PDF]
|
|