| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
© 1994 Oxford University Press
other |
DNA single-strand breaks in kidneys of Syrian hamsters treated with steroidal estrogens: hormone-induced free radical damage preceding renal malignancy
Department of Pharmacology and Toxicology, The University of Texas Medical Branch Galveston, TX 77555-1031, USA
1To whom correspondence should be addressed
The chronic administration of estradiol by subcutaneous (s.c.) implantation into male Syrian hamsters induces kidney tumors. Free radicals generated by redox cycling between catecholestrogens and their quinones have been proposed to damage DNA and to thus mediate renal hormone-induced carcinogenesis. As part of an examination of this postulate, we assayed by a filter elution technique DNA single-strand breaks in livers and kidneys of male hamsters treated with estrogen by single intraperitoneal (i.p.) injection, by s.c. implant or by continuous infusion and compared values to those in untreated controls. The DNAs of hamster liver and kidney were not affected by one i.p. injection of 5, 15 or 150 mg/kg estradiol. However, treatment of hamsters with one 25 mg estradiol lmplantlanimal for 2 weeks elevated by 10% the levels of DNA single-strand breaks in kidney, but only to a minor extent in liver, which is not a target of estrogen- induced carcinogenesis. An infusion of 250 µg/day/animal of estradiol or 4-hydroxyestradiol for one week by osmotic pumps into hamsters resulted in a comparable increase of single-strand breaks in kidney DNA, whereas 2-hydroxyestra- diol under these conditions had a negligible effect. It is concluded that the induction of DNA single-strand breakage by either estradlol or 4-hydroxyestradiol in hamster kidney supports a mechanism of estrogen-Induced carcinogenesis by free radical generation via redox cycling between 4-hydroxy- estradiol and Its corresponding quinone.