Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (19)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Bianchini, F.
Right arrow Articles by Wild, C. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bianchini, F.
Right arrow Articles by Wild, C. P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1994 Oxford University Press

research-article

Comparison of 7-medG formation in white blood cells, liver and target organs in rats treated with methylating carcinogens

Franca Bianchini and Christopher P. Wild 1

International Agency for Research on Cancer 150 Cours Albert Thomas, 69372 Lyon Cedex 08, France

1To whom reprint requests should be sent

The relationship between 7-methylguanine (7-meG) formation in white blood cells (WBC) and internal organs after treatment with methylating carcinogens has been studied in rats, In order to assess the possible use of WBC as a surrogate for target organs in epidemiological studies. Animals were treated with a single intraperitoneal injection of DMN, DMH or NMBA or with a subcutaneous injection of NNK, at different dose levels, and killed 16 h after treatment. 7-meG, following thermal hydrolysis and immunopurification, was analysed by HPLC with electrochemical detection. Administration of methylating carcinogens resulted in the dose-dependent formation of 7-meG in WBC DNA; the adduct level, however, was markedly lower than in internal organs. The ratio between 7-meG formation in target organ and WBC was a function of the carcinogen administered, varying from 3.6 with NNK to 200 with NMBA. On the other hand, the ratio between 7-meG in the liver and WBC was of the same order of magnitude for each carcinogen, ranging from 35 to 100. These results show that the presence of 7-meG in WBC DNA is indicative of adduct formation in internal organs, particularly in the liver, and suggest that active methylating species may be formed in the liver and then transferred to the WBC where they can methylate DNA.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Toxicol PatholHome page
A. C. Povey
DNA Adducts: Endogenous and Induced
Toxicol Pathol, May 1, 2000; 28(3): 405 - 414.
[Abstract] [PDF]

Home page
 CarcinogenesisHome page
R. W.L. Godschalk, E. J.C. Moonen, P. A.E.L. Schilderman, W. M.R. Broekmans, J. C.S. Kleinjans, and F. J. Van Schooten
Exposure-route-dependent DNA adduct formation by polycyclic aromatic hydrocarbons
Carcinogenesis, January 1, 2000; 21(1): 87 - 92.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.