© 1995 Oxford University Press
research-article |
Effect of ß-carotene and wheat bran fiber on colonic aberrant crypt and tumor formation in rats exposed to azoxymethane and high dietary fat
Institute for Disease Prevention 421 Ross Hall, Washington, DC 20037, USA
1Department of Pathology, George Washington University Medical Center 421 Ross Hall, Washington, DC 20037, USA
The typical high-fat, low-fiber American diet promotes colon cancer. An alternative to radical changes in dietary habits is to reinforce the diet with cancer protectors. Experiments to evaluate the effects of ß-carotene in the presence of high fat and low and high dietary levels of wheat bran fiber were designed using the Fischer-344 rat colon cancer model. Rats (20/group), were given either high fat (20% w/w), low wheat bran fiber (1% w/w) diets, or high fat (20% w/w) high wheat bran fiber (8% w/w) diets, with different levels of ß-carotene. After 2 weeks of adaptation, half were given two weekly s.c. injections of azoxymethane (AOM, 15 mg/kg body wt); and half two weekly s.c. injections of saline. Six weeks later, five rats from each dietary group were killed to evaluate the comparative effect of different dietary regimens on the induction of colon aberrant crypt foci (ACF). The remaining rats were maintained on their respective diets for an additional 20 weeks to examine the effect on colon tumor incidence. The total number of ACE/rat in the low-fiber groups declined from 44.0 ± 4.18 to 12.8 ± 1.95 in response to increasing amounts of ß-carotene from 1 to 20 mg/kg diet. A similar progressive reduction in total ACF/rat was also seen in the high-fiber groups (20.8 ± 2.92 to 9.2 ± 0.58). ACF did not develop in the saline-exposed groups. Similarly colon tumor incidence declined from 73% to 20% in high-fiber groups and from 27% to 13% in low-fiber groups In response to increasing amounts ß-carotene from 1 to 20 mg/kg diet. The results showed that ß-carotene and wheat bran, individually and when combined, protected the colon in rats consuming high-fat, western-style diets from ACF and benign or malignant tumor formation.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Qu, R. L. Madl, D. J. Takemoto, R. C. Baybutt, and W. Wang Lignans Are Involved in the Antitumor Activity of Wheat Bran in Colon Cancer SW480 Cells J. Nutr., March 1, 2005; 135(3): 598 - 602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Palozza, S. Serini, N. Maggiano, G. Tringali, P. Navarra, F. O. Ranelletti, and G. Calviello {beta}-Carotene Downregulates the Steady-State and Heregulin-{alpha}-Induced COX-2 Pathways in Colon Cancer Cells J. Nutr., January 1, 2005; 135(1): 129 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-K. Hsu, J.-W. Liao, Y.-C. Chung, C.-P. Hsieh, and Y.-C. Chan Xylooligosaccharides and Fructooligosaccharides Affect the Intestinal Microbiota and Precancerous Colonic Lesion Development in Rats J. Nutr., June 1, 2004; 134(6): 1523 - 1528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Mollersen, J. E. Paulsen, H. B. Olstorn, H. K. Knutsen, and J. Alexander Dietary retinoic acid supplementation stimulates intestinal tumour formation and growth in multiple intestinal neoplasia (Min)/+ mice Carcinogenesis, January 1, 2004; 25(1): 149 - 153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Palozza, S. Serini, N. Maggiano, M. Angelini, A. Boninsegna, F. Di Nicuolo, F. O. Ranelletti, and G. Calviello Induction of cell cycle arrest and apoptosis in human colon adenocarcinoma cell lines by {beta}-carotene through down-regulation of cyclin A and Bcl-2 family proteins Carcinogenesis, January 1, 2002; 23(1): 11 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.V.W. Wijnands, H.C. Schoterman, J.P. Bruijntjes, V.M.H. Hollanders, and R.A. Woutersen Effect of dietary galacto-oligosaccharides on azoxymethane-induced aberrant crypt foci and colorectal cancer in Fischer 344 rats Carcinogenesis, January 1, 2001; 22(1): 127 - 132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. H. McIntosh, P. J. Royle, and G. Pointing Wheat Aleurone Flour Increases Cecal {beta}-Glucuronidase Activity and Butyrate Concentration and Reduces Colon Adenoma Burden in Azoxymethane-Treated Rats J. Nutr., January 1, 2001; 131(1): 127 - 131. [Abstract] [Full Text]
|
|
|
|
|
|
M. Jenab and L. U. Thompson Phytic acid in wheat bran affects colon morphology, cell differentiation and apoptosis Carcinogenesis, August 1, 2000; 21(8): 1547 - 1552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Rijken, W. G. Timmer, A. J. van de Kooij, I. M. van Benschop, S. A. Wiseman, M. Meijers, and L. B.M. Tijburg Effect of vegetable and carotenoid consumption on aberrant crypt multiplicity, a surrogate end-point marker for colorectal cancer in azoxymethane-induced rats Carcinogenesis, December 1, 1999; 20(12): 2267 - 2272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Gallaher and J. Khil The Effect of Synbiotics on Colon Carcinogenesis in Rats J. Nutr., July 1, 1999; 129(7): 1483 - 1483. [Abstract] [Full Text]
|
|
|
|
|
|
M. J. Davies, E. A. Bowey, H. Adlercreutz, I. R. Rowland, and P. C. Rumsby Effects of soy or rye supplementation of high-fat diets on colon tumour development in azoxymethane-treated rats Carcinogenesis, June 1, 1999; 20(6): 927 - 931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ishizuka, S. Ito, M. Onuma, T. Kasai, Y. Aoyama, and H. Hara Ingestion of sugar beet fiber enhances irradiation-induced aberrant crypt foci in the rat colon under an apoptosis-suppressed condition Carcinogenesis, June 1, 1999; 20(6): 1005 - 1009. [Abstract] [Full Text] [PDF]
|
|