© 1995 Oxford University Press
research-article |
cDNA and genomic sequences for rat 8-oxo-dGTPase that prevents occurrence of spontaneous mutations due to oxidation of guanine nucleotides
Department of Biochemistry, Medical Institute of Bioregulation, Kyushu University 69, Fukuoka 8 12-82, Japan
1To whom correspondence should be addressed
The enzyme, 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase (8-oxo-dGTPase), is present In various organisms and plays an important role in control of spontaneous mutagenesis. This enzyme degrades 8-oxoguamne-contaning deoxyribonucleoside triphosphate, a potentially mutagenic substrate for DNA synthesis, to the corresponding monophosphate. To obtain appropriate probes for expression of the gene in various tissues and also to construct appropriate experimental models for carcino genesis, we cloned cDNA for rat 8-oxo-dGTPase and elucidated its structure. The nucleotide sequence of the cDNA revealed that the rat 8-oxo-dGTPase protein is composed of 156 amino acid residues. The molecular weight of rat 8-oxo-dGTPase, calculated from the predicted amino acid se was 18 006, and the 8-oxo-dGTPase protein of this size was detected when the cDNA was expressed in 8-oxo-dGTPase-deficlent Escherichitz coil mutT– cells. The predicted amino acid sequence of the rat 8-oxo-dGTPase has a close homology with those of human and bacterial counterparts. Using the cDNA as a probe, part of the rat gene for 8-oxo-dGTPase was isolated and was found to consist of at least three exons and spanned about 10 kb. A genomic region containing the pseudogene was also isolated.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Ogawa, Y. Ueda, K. Yoshimura, and S. Shigeoka Comprehensive Analysis of Cytosolic Nudix Hydrolases in Arabidopsis thaliana J. Biol. Chem., July 1, 2005; 280(26): 25277 - 25283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mishima, Y. Sakai, N. Itoh, H. Kamiya, M. Furuichi, M. Takahashi, Y. Yamagata, S. Iwai, Y. Nakabeppu, and M. Shirakawa Structure of Human MTH1, a Nudix Family Hydrolase That Selectively Degrades Oxidized Purine Nucleoside Triphosphates J. Biol. Chem., August 6, 2004; 279(32): 33806 - 33815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Yoshimura, K. Sakumi, M. Ohno, Y. Sakai, M. Furuichi, S. Iwai, and Y. Nakabeppu An Oxidized Purine Nucleoside Triphosphatase, MTH1, Suppresses Cell Death Caused by Oxidative Stress J. Biol. Chem., September 26, 2003; 278(39): 37965 - 37973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang, Y. Zhang, and M. Inouye Thermotoga maritima MazG Protein Has Both Nucleoside Triphosphate Pyrophosphohydrolase and Pyrophosphatase Activities J. Biol. Chem., June 6, 2003; 278(24): 21408 - 21414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sakai, M. Furuichi, M. Takahashi, M. Mishima, S. Iwai, M. Shirakawa, and Y. Nakabeppu A Molecular Basis for the Selective Recognition of 2-Hydroxy-dATP and 8-Oxo-dGTP by Human MTH1 J. Biol. Chem., March 1, 2002; 277(10): 8579 - 8587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Tsuzuki, A. Egashira, H. Igarashi, T. Iwakuma, Y. Nakatsuru, Y. Tominaga, H. Kawate, K. Nakao, K. Nakamura, F. Ide, et al. Spontaneous tumorigenesis in mice defective in the MTH1 gene encoding 8-oxo-dGTPase PNAS, September 25, 2001; 98(20): 11456 - 11461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Shimokawa, Y. Fujii, M. Furuichi, M. Sekiguchi, and Y. Nakabeppu Functional significance of conserved residues in the phosphohydrolase module of Escherichia coli MutT protein Nucleic Acids Res., September 1, 2000; 28(17): 3240 - 3249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ohtsubo, K. Nishioka, Y. Imaiso, S. Iwai, H. Shimokawa, H. Oda, T. Fujiwara, and Y. Nakabeppu Identification of human MutY homolog (hMYH) as a repair enzyme for 2-hydroxyadenine in DNA and detection of multiple forms of hMYH located in nuclei and mitochondria Nucleic Acids Res., March 15, 2000; 28(6): 1355 - 1364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Fujii, H. Shimokawa, M. Sekiguchi, and Y. Nakabeppu Functional Significance of the Conserved Residues for the 23-Residue Module among MTH1 and MutT Family Proteins J. Biol. Chem., December 31, 1999; 274(53): 38251 - 38259. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Bialkowski, A. Bialkowska, and K. S. Kasprzak Cadmium(II), unlike nickel(II), inhibits 8-oxo-dGTPase activity and increases 8-oxo-dG level in DNA of the rat testis, a target organ for cadmium(II) carcinogenesis Carcinogenesis, August 1, 1999; 20(8): 1621 - 1624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kobayashi, Y. Ohara-Nemoto, M. Kaneko, H. Hayakawa, M. Sekiguchi, and K. Yamamoto Potential of Escherichia coli GTP Cyclohydrolase II for Hydrolyzing 8-Oxo-dGTP, a Mutagenic Substrate for DNA Synthesis J. Biol. Chem., October 9, 1998; 273(41): 26394 - 26399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sheikh, S. F. O'Handley, C. A. Dunn, and M. J. Bessman Identification and Characterization of the Nudix Hydrolase from the Archaeon, Methanococcus jannaschii, as a Highly Specific ADP-ribose Pyrophosphatase J. Biol. Chem., August 14, 1998; 273(33): 20924 - 20928. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Takahashi, M. Hirose, S. Tamano, M. Ozaki, S.-I. Orita, T. Ito, M. Takeuchi, H. Ochi, S. Fukada, H. Kasai, et al. Immunohistochemical Detection of 8-Hydroxy-2'-deoxyguanosine in Paraffin-Embedded Sections of Rat Liver after Carbon Tetrachloride Treatment Toxicol Pathol, March 1, 1998; 26(2): 247 - 252. [Abstract] [PDF]
|
|
|
|
|
|
S. F. O'Handley, D. N. Frick, C. A. Dunn, and M. J. Bessman Orf186 Represents a New Member of the Nudix Hydrolases, Active on Adenosine(5')triphospho(5')adenosine, ADP-ribose, and NADH J. Biol. Chem., February 6, 1998; 273(6): 3192 - 3197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. C. Kuo and J. Sklar Augmented Expression of a Human Gene for 8-oxoguanine DNA Glycosylase (MutM) in B Lymphocytes of the Dark Zone in Lymph Node Germinal Centers J. Exp. Med., November 3, 1997; 186(9): 1547 - 1556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Taddei, H. Hayakawa, M. Bouton, A. Cirinesi, I. Matic, M. Sekiguchi, and M. Radman Counteraction by MutT Protein of Transcriptional Errors Caused by Oxidative Damage Science, October 3, 1997; 278(5335): 128 - 130. [Abstract] [Full Text]
|
|
|
|
|
|
H. Oda, Y. Nakabeppu, M. Furuichi, and M. Sekiguchi Regulation of Expression of the Human MTH1 Gene Encoding 8-Oxo-dGTPase. ALTERNATIVE SPLICING OF TRANSCRIPTION PRODUCTS J. Biol. Chem., July 11, 1997; 272(28): 17843 - 17850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Igarashi, T. Tsuzuki, T. Kakuma, Y. Tominaga, and M. Sekiguchi Organization and Expression of the Mouse MTH1 Gene for Preventing Transversion Mutation J. Biol. Chem., February 7, 1997; 272(6): 3766 - 3772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Bessman, D. N. Frick, and S. F. O'Handley The MutT Proteins or ``Nudix'' Hydrolases, a Family of Versatile, Widely Distributed, ``Housecleaning'' Enzymes J. Biol. Chem., October 11, 1996; 271(41): 25059 - 25062. [Full Text] [PDF]
|
|
|
|
|
|
S. F. O'Handley, C. A. Dunn, and M. J. Bessman Orf135 from Escherichia coli Is a Nudix Hydrolase Specific for CTP, dCTP, and 5-Methyl-dCTP J. Biol. Chem., February 16, 2001; 276(8): 5421 - 5426. [Abstract] [Full Text] [PDF]
|
|