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p53 Expression in normal versus transformed mammalian cells
1CSTA-Mutagenesis Laboratory, National Institute for Research on Cancer IST, V.le Benedetto XV 10, 16132 Genoa
2Institute of Mutagenesis and Differentiation of CNR Via Svezia 10, 56100 Pisa
3Chair of Genetics, University of Genoa C.so Europa, 16132 Genoa, Italy
To answer the question whether the level of p53 expression also reflects the status of a cell, with reference to trans formation and genome stability, we have examined, by Immunocytochemistry, the presence of p53 protem in a number of cell types including human diploid cells, Chinese hamster embryonal cells at different passages and gene amplilled and/or transformed Chinese hamster cell lines. Primary human fibroblasts at early passage (LEO) and an established, non transformed, Chinese hamster cell line at early passage (CHIEF/18) did not show any detectable p53 expression, either nuclear or cytoplasmic. All transformed human (Raji) and Chinese hamster cell lines (CHO, V79, V79/B7) showed a nuclear expression of p53, although at different intensities. Two cell lines selected from V79/ B7 for their resistance to phosphonacetyl-L-aspartate or methotrexate and previously shown to bear gene amplification, showed p53 expressIon. In PALA L cells p53 expressIon was nuclear as in other positive cell lines tested, while in MTX M cells it was cytoplasmlc. CHEF/18 cells at late passage in culture showed the typical behaviour of trans formed cells and p53 was detected in several cells. Moreover, when transformed CHO cells were treated with compounds known to induce reverse transformation, both the disap pearance of hallmarks of transformed phenotype and p53 reduction were observed. These results indicate a strong association within the same cell type between p53 expression and transformed status.
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