© 1996 Oxford University Press
research-article |
Diallyl disulfide induces apoptosis of human colon tumor cells
Department of Nutrition. The Pennsylvania State University University Park, PA 16802. USA
1To whom correspondence should be addressed
The present studies compared the effects of various oil-soluble compounds containing allyl and disulfide groups on the proliferation of cultured human colon tumor cells (HCT-15). Diallyl disulfide (DADS) was more effective in inhibiting the growth of HCT-15 cells than isomolar concentrations of S-allyl cysteine, dipropyl disulfide (DPDS), allyl chloride, allyl glycidyl ether and allyl alcohol. These studies clearly demonstrate the importance of both the diallyl and the disulfide groups in DADS. Treatment of HCT-15 cells with 100 µM DADS increased the intracellular calcium levels by 40%,while DPDS caused only a 12% increase in intracellular calcium. Exposure to 100 µM DADS or more, but not DPDS, caused the cells to undergo apoptosis as determined by morphological changes and DNA fragmentation. A positive correlation (r = 0.944) was found between DADS-induced DNA fragmentation and its ability to increase intracellular free calcium levels. The widespread effectiveness of DADS was evident by its ability to inhibit the growth of human colon (HCT-15), skin (SKMEL-2) and lung (A549) tumor cell lines.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. V. Singh, A. A. Powolny, S. D. Stan, D. Xiao, J. A. Arlotti, R. Warin, E.-R. Hahm, S. W. Marynowski, A. Bommareddy, D. M. Potter, et al. Garlic Constituent Diallyl Trisulfide Prevents Development of Poorly Differentiated Prostate Cancer and Pulmonary Metastasis Multiplicity in TRAMP Mice Cancer Res., November 15, 2008; 68(22): 9503 - 9511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. T. Ngo, D. B. Williams, L. Cobiac, and R. J. Head Does Garlic Reduce Risk of Colorectal Cancer? A Systematic Review J. Nutr., October 1, 2007; 137(10): 2264 - 2269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-A. Kim, D. Xiao, H. Xiao, A. A. Powolny, K. L. Lew, M. L. Reilly, Y. Zeng, Z. Wang, and S. V. Singh Mitochondria-mediated apoptosis by diallyl trisulfide in human prostate cancer cells is associated with generation of reactive oxygen species and regulated by Bax/Bak Mol. Cancer Ther., May 1, 2007; 6(5): 1599 - 1609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Herman-Antosiewicz, S. D. Stan, E.-R. Hahm, D. Xiao, and S. V. Singh Activation of a novel ataxia-telangiectasia mutated and Rad3 related/checkpoint kinase 1-dependent prometaphase checkpoint in cancer cells by diallyl trisulfide, a promising cancer chemopreventive constituent of processed garlic Mol. Cancer Ther., April 1, 2007; 6(4): 1249 - 1261. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Xiao, K. L. Lew, Y.-A. Kim, Y. Zeng, E.-R. Hahm, R. Dhir, and S. V. Singh Diallyl Trisulfide Suppresses Growth of PC-3 Human Prostate Cancer Xenograft In vivo in Association with Bax and Bak Induction. Clin. Cancer Res., November 15, 2006; 12(22): 6836 - 6843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Antosiewicz, A. Herman-Antosiewicz, S. W. Marynowski, and S. V. Singh c-Jun NH2-Terminal Kinase Signaling Axis Regulates Diallyl Trisulfide-Induced Generation of Reactive Oxygen Species and Cell Cycle Arrest in Human Prostate Cancer Cells. Cancer Res., May 15, 2006; 66(10): 5379 - 5386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Xiao and S. V. Singh Diallyl trisulfide, a constituent of processed garlic, inactivates Akt to trigger mitochondrial translocation of BAD and caspase-mediated apoptosis in human prostate cancer cells Carcinogenesis, March 1, 2006; 27(3): 533 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-j. Wu, T. Stahl, Y. Hu, F. Kassie, and V. Mersch-Sundermann The Production of Reactive Oxygen Species and the Mitochondrial Membrane Potential Are Modulated during Onion Oil-Induced Cell Cycle Arrest and Apoptosis in A549 Cells J. Nutr., March 1, 2006; 136(3): 608 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Katsuki, K. Hirata, H. Ishikawa, N. Matsuura, S.-i. Sumi, and H. Itoh Aged Garlic Extract Has Chemopreventative Effects On 1,2-Dimethylhydrazine-Induced Colon Tumors in Rats J. Nutr., March 1, 2006; 136(3): 847S - 851S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Xiao, J. T. Pinto, G. G. Gundersen, and I. B. Weinstein Effects of a series of organosulfur compounds on mitotic arrest and induction of apoptosis in colon cancer cells Mol. Cancer Ther., September 1, 2005; 4(9): 1388 - 1398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Herman-Antosiewicz and S. V. Singh Checkpoint Kinase 1 Regulates Diallyl Trisulfide-induced Mitotic Arrest in Human Prostate Cancer Cells J. Biol. Chem., August 5, 2005; 280(31): 28519 - 28528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Knowles and J. A. Milner Diallyl Disulfide Induces ERK Phosphorylation and Alters Gene Expression Profiles in Human Colon Tumor Cells J. Nutr., September 1, 2003; 133(9): 2901 - 2906. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. A. Hamill Colon Cancer and the Western Diet Integr Cancer Ther, December 1, 2002; 1(4): 420 - 424. [PDF]
|
|
|
|
|
|
C. Bose, J. Guo, L. Zimniak, S. K. Srivastava, S. P. Singh, P. Zimniak, and S. V. Singh Critical role of allyl groups and disulfide chain in induction of Pi class glutathione transferase in mouse tissues in vivo by diallyl disulfide, a naturally occurring chemopreventive agent in garlic Carcinogenesis, October 1, 2002; 23(10): 1661 - 1665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. G. Bottone Jr., S. J. Baek, J. B. Nixon, and T. E. Eling Diallyl Disulfide (DADS) Induces the Antitumorigenic NSAID-Activated Gene (NAG-1) by a p53-Dependent Mechanism in Human Colorectal HCT 116 Cells J. Nutr., April 1, 2002; 132(4): 773 - 778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Robert, B. Mouille, C. Mayeur, M. Michaud, and F. Blachier Effects of the garlic compound diallyl disulfide on the metabolism, adherence and cell cycle of HT-29 colon carcinoma cells: evidence of sensitive and resistant sub-populations Carcinogenesis, August 1, 2001; 22(8): 1155 - 1161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Nakagawa, K. Tsuta, K. Kiuchi, H. Senzaki, K. Tanaka, K. Hioki, and A. Tsubura Growth inhibitory effects of diallyl disulfide on human breast cancer cell lines Carcinogenesis, June 1, 2001; 22(6): 891 - 897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Milner A Historical Perspective on Garlic and Cancer J. Nutr., March 1, 2001; 131(3): 1027S - 1031. [Abstract] [Full Text]
|
|
|
|
|
|
L. M. Knowles and J. A. Milner Possible Mechanism by Which Allyl Sulfides Suppress Neoplastic Cell Proliferation J. Nutr., March 1, 2001; 131(3): 1061S - 1066. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Teyssier and M.-H. Siess Metabolism of Dipropyl Disulfide by Rat Liver Phase I and Phase II Enzymes and by Isolated Perfused Rat Liver Drug Metab. Dispos., June 1, 2000; 28(6): 648 - 654. [Abstract] [Full Text]
|
|
|
|
|
|
L. M. Knowles and J. A. Milner Diallyl disulfide inhibits p34cdc2 kinase activity through changes in complex formation and phosphorylation Carcinogenesis, June 1, 2000; 21(6): 1129 - 1134. [Abstract] [Full Text] [PDF]
|
|