Carcinogenesis, Vol 18, 83-88, Copyright © 1997 by Oxford University Press
MT Huang, W Ma, P Yen, JG Xie, J Han, K Frenkel, D Grunberger and AH Conney
The effects of topical applications of very low doses of curcumin (the
major yellow pigment in turmeric and the Indian food curry) on 12-O-
tetradecanoylphorbol-13-acetate (TPA)-induced oxidation of DNA bases in the
epidermis and on tumor promotion in mouse skin were investigated. CD-1 mice
were treated topically with 200 nmol of 7,12- dimethylbenz[a]anthracene
followed one week later by 5 nmol of TPA alone or together with 1, 10, 100
or 3000 nmol of curcumin twice a week for 20 weeks. Curcumin-mediated
effects on TPA-induced formation of the oxidized DNA base
5-hydroxymethyl-2'-deoxyuridine (HMdU) and tumor formation were determined.
All dose levels of curcumin inhibited the mean values of TPA-induced HMdU
formation in epidermal DNA (62-77% inhibition), but only the two highest
doses of curcumin strongly inhibited TPA-induced tumor promotion (62-79%
inhibition of tumors per mouse and tumor volume per mouse). In a second
experiment, topical application of 20 or 100 nmol (but not 10 nmol) of
curcumin together with 5 nmol TPA twice a week for 18 weeks markedly
inhibited TPA- induced tumor promotion. Curcumin had a strong inhibitory
effect on DNA and RNA synthesis (IC50 = 0.5-1 microM) in cultured HeLa
cells, but there was little or no effect on protein synthesis.
ARTICLES
Inhibitory effects of topical application of low doses of curcumin on 12-O-tetradecanoylphorbol-13-acetate-induced tumor promotion and oxidized DNA bases in mouse epidermis
Department of Chemical Biology, College of Pharmacy, Rutgers, The State University of New Jersey, Piscataway 08855-0789, USA.
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