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Carcinogenesis, Vol 18, 1965-1972, Copyright © 1997 by Oxford University Press

ARTICLES

Computerized image analysis of morphologically transformed and nontransformed Syrian hamster embryo (SHE) cell colonies: application to objective SHE cell transformation assay scoring

GM Ridder, SB Stuard, GA Kerckaert, DB Cody, RA LeBoeuf and RJ Isfort
CP&RSD/HSD, The Procter & Gamble Company, Miami Valley Laboratories, Cincinnati, OH 45253-8707, USA.

We have developed an automated image analysis system that provides comparable classification of morphologically transformed SHE cell colonies to the current visual classification method used in the in vitro SHE cell transformation assay. Visual classification of morphologic transformation in this assay has been shown to accurately predict the carcinogenic potential of chemical, biological and physical agents. The image analysis system is quantitative, based on measuring features of colony color, texture and growth patterns. A linear combination of feature measurements produces a classification process that agrees with visual assessment 93% of the time. All identifiable sources of error are explored and the method is found to be robust in analyzing nearly 500 colonies from a variety of studies performed over a one year period. The high degree of correlation between the visual classification and the objective measurements of the image analysis system validates the reproducibility of the visual scoring process and serves as a basis for automation of the assay.
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