Carcinogenesis, Vol 18, 2163-2169, Copyright © 1997 by Oxford University Press
YP Lu, YR Lou, JG Xie, P Yen, MT Huang and AH Conney
Female CD-1 mice were initiated with a single topical application of
7,12-dimethylbenz[a]anthracene and promoted with 12-O-
tetradecanoylphorbol-13-acetate. Mice with established papillomas were then
treated with black tea or decaffeinated black tea (approximately 4 mg tea
solids/ml) as the sole source of drinking fluid for 11-15 weeks. In four
separate experiments, oral administration of black tea inhibited the growth
of papillomas (increase in tumor volume/mouse) by an average of 35%, 37%,
41% and 48%, respectively. Studies with decaffeinated black tea gave
inconsistent results. In one experiment, administration of decaffeinated
black tea inhibited papilloma growth (increase in tumor volume/mouse) by
27%, but in two additional experiments papilloma growth was stimulated by
14% and 193%, respectively. In a separate experiment, skin tumors were
generated by treating SKH-1 female mice with ultraviolet B light (UVB; 30
mJ/cm2) twice weekly for 22 weeks, after which UVB administration was
stopped. Tumors were allowed to develop during the following 13 weeks, and
tumor- bearing mice were then treated with black tea (6 mg/ml tea solids)
as the drinking fluid for 11 weeks. In this experiment, tumor growth
(increase in tumor volume/mouse) was inhibited by 70%. Histological
examination revealed that tea-treated mice had a 58% decrease in the number
of nonmalignant tumors (primarily keratoacanthomas)/mouse and a 54%
decrease in the number of squamous cell carcinomas/mouse. In addition,
administration of black tea decreased the volume per tumor by 60% for
nonmalignant tumors and by 84% for carcinomas. Mechanistic studies with
tumors from these mice revealed that administration of black tea decreased
the bromodeoxyuridine labeling index in squamous cell papillomas,
keratoacanthomas and squamous cell carcinomas by 56%, 45% and 35%,
respectively, and the apoptosis index was increased by 44%, 100% and 95%,
respectively. Administration of black tea decreased the mitotic index in
keratoacanthomas and squamous cell carcinomas by 42% and 16%, respectively.
The results indicate that oral administration of black tea to tumor-bearing
mice inhibited proliferation and enhanced apoptosis in nonmalignant and
malignant skin tumors.
ARTICLES
Inhibitory effect of black tea on the growth of established skin tumors in mice: effects on tumor size, apoptosis, mitosis and bromodeoxyuridine incorporation into DNA
Department of Chemical Biology, College of Pharmacy, Rutgers, The State University of New Jersey, Piscataway 08855-0789, USA.
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