Carcinogenesis, Vol 18, 339-344, Copyright © 1997 by Oxford University Press
M Peluso, E Amasio, S Bonassi, A Munnia, F Altrupa and S Parodi
Nasal epithelium is an easily accessible tissue that is potentially useful
for human biomonitoring studies aimed at evaluating exposure to airborne
carcinogens. We have devised a simple technique, which causes minimum
distress to the informed patient, to obtain very small but sufficient
biopsies from the inferior or middle turbinate head. DNA adducts were
measured by 32P-postlabeling assay in nasal mucosa of nine cigarette
smokers (including two subjects who had given up smoking shortly before
sampling), two former smokers and 10 non-smoker healthy donors. None of the
subjects reported other recent exposures to mutagens or carcinogens. Using
the nuclease P1 technique, a mean adduct level of 4.8/10(8) bases and a
specific spot pattern, the diagonal radioactive zone, were found in
smokers, whereas non-smokers showed a significantly lower global level of
DNA adducts, i.e. 1.4/10(8) bases, and no diagonal zone. Another important
result was the presence of a significant association between DNA adduct
level and the number of cigarettes smoked daily. These preliminary findings
suggest that the level of DNA adducts measured from biopsies of the nasal
mucosa is a reliable marker of exposure to cigarette smoking and uphold its
use in biomonitoring exposures to other airborne DNA binding compounds.
ARTICLES
Detection of DNA adducts in human nasal mucosa tissue by 32P- postlabeling analysis
Servizio di Oncologia Sperimentale, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
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