Carcinogenesis, Vol 18, 811-816, Copyright © 1997 by Oxford University Press
C Kielbassa, L Roza and B Epe
DNA damage induced by UV radiation and visible light (290-500 nm) in AS52
Chinese hamster cells was analysed by an alkaline elution assay with
specific repair endonucleases. Cells were exposed to extensively filtered
monochrome or broad-band radiation. Between 290 and 315 nm, the ratio of
base modifications sensitive to Fpg protein (i.e. 8- hydroxyguanine and
formamidopyrimidines) and T4 endonuclease V (i.e. cyclobutane pyrimidine
dimers) was constant (approximately 1:200), indicating that the direct
excitation of DNA is responsible for both types of damage in this range of
the spectrum. While the yield of pyrimidine dimers per unit dose continued
to decrease exponentially beyond 315 nm, the yield of Fpg-sensitive
modifications increased to a second maximum between 400 and 450 nm. The
damage spectrum in this wavelength range consisted of only a few other
modifications (strand breaks, abasic sites and pyrimidine modifications
sensitive to endonuclease III) and is attributed to endogenous
photosensitizers that give rise to oxidative DNA damage via singlet oxygen
and/or type I reactions. The generation of Fpg-sensitive modifications by
visible light was not linear with dose but followed a saturation curve. It
is calculated that the exposure of the cells to low doses of solar
radiation results in the formation of cyclobutane pyrimidine dimers and
Fpg-sensitive modifications in a ratio of 10:1.
ARTICLES
Wavelength dependence of oxidative DNA damage induced by UV and visible light
Institute of Pharmacy, University of Mainz, Germany.
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