Carcinogenesis, Vol 18, 1319-1328, Copyright © 1997 by Oxford University Press
M Saitoh, M Oyamada, Y Oyamada, T Kaku and M Mori
We examined changes in the expression and localization of connexin proteins
and transcripts by means of immunofluorescence and in situ hybridization in
normal conditions, wound healing and carcinogenesis using hamster tongue
epithelium, in which differentiation, migration and growth of keratinocytes
takes place physiologically and pathologically. In normal hamster tongue
epithelium, immunofluorescent staining showed that Cx26 and Cx43 proteins
were localized differently during differentiation of keratinocytes, but in
in situ hybridization, the localization of Cx26 and Cx43 transcripts
overlapped considerably, suggesting that the different localization of Cx26
and Cx43 proteins in squamous epithelium is largely regulated at
post-transcriptional levels. During wound healing, the expression and
localization of connexin proteins and transcripts were changed drastically.
Shortly (6 h) after injury the expression of Cx26 and Cx43 proteins
decreased at wound edges, but by 1-3 days after injury the expression of
both proteins increased and both proteins co-localized to the same spots in
the epithelium near wound edges. During carcinogenesis, the increased
expression of Cx26 and Cx43 proteins and their transcripts and co-
localization of both proteins occurred in papillomas, and the expression of
Cx26 was reduced as cancer cells became morphologically less
differentiated. We also found, that during wound healing in papillomas,
squamous cell carcinomas and keratinocytes, Cx26 and Cx43 proteins were
localized aberrantly in the cytoplasm, especially around nuclei, rather
than on plasma membranes. These results indicate that quantitative and
qualitative changes in connexin expression are associated with
differentiation, migration and proliferation of keratinocytes in squamous
epithelium.
ARTICLES
Changes in the expression of gap junction proteins (connexins) in hamster tongue epithelium during wound healing and carcinogenesis
Department of Pathology, Sapporo Medical University School of Medicine, Chuo-ku, Japan.
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