Carcinogenesis, Vol 18, 1847-1850, Copyright © 1997 by Oxford University Press
BL Pool-Zobel, A Bub, H Muller, I Wollowski and G Rechkemmer
A human intervention study with vegetable products has been performed in
twenty three healthy, non smoking males aged 27-40. It was the aim of the
study to assess whether consumption of vegetables containing different
carotenoids could protect against DNA damage and oxidative DNA damage. The
subjects consumed their normal diets, but abstained from vegetables high in
carotenoids throughout the study period. After a 2 week depletion period,
they received daily 330 ml tomato juice with 40 mg lycopene (weeks 3 and
4), 330 ml carrot juice with 22.3 mg beta- carotene and 15.7 mg
alpha-carotene (weeks 5 and 6), and 10 g dried spinach powder (in water or
milk) with 11.3 mg lutein (weeks 7 and 8). Blood was collected weekly and
DNA damage was detected in peripheral blood lymphocytes with the 'COMET'
assay. Oxidised DNA bases were detected by including an incubation step
with endonuclease III. The supplementation of the diet with tomato, carrot
or spinach products resulted in a significant decrease in endogenous levels
of strand breaks in lymphocyte DNA. Oxidative base damage was significantly
reduced during the carrot juice intervention. These findings support the
hypothesis that carotenoid containing plant products exert a cancer-
protective effect via a decrease in oxidative and other damage to DNA in
humans.
ARTICLES
Consumption of vegetables reduces genetic damage in humans: first results of a human intervention trial with carotenoid-rich foods
Institute of Nutritional Physiology, Federal Research Centre for Nutrition, Karlsruhe, Germany.
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