Carcinogenesis, Vol 19, 61-67, Copyright © 1998 by Oxford University Press
K Cesen-Cummings, MJ Fernstrom, AM Malkinson and RJ Ruch
The reduced gap junctional intercellular communication (GJIC) and gap
junction protein (connexin) expression that have been noted in many
neoplastic cell types may contribute to the neoplastic phenotype. We
assessed GJIC (by fluorescent dye micro-injection) and connexin expression
(by Northern blotting, Western blotting and immunohistochemistry) in five
mouse and 17 human lung carcinoma cell lines; both measures were lower in
neoplastic cells compared to non- transformed lung epithelial cells. Other
connexins were not detected in these cells. Co-culture experiments
indicated that carcinoma cell lines able to transfer dye among themselves
(homologous GJIC) had little capacity for dye-coupling with non-transformed
cells (heterologous GJIC). Southern blot analyses indicated that reductions
in GJIC and connexin43 expression were not due to deletions or
rearrangements of this gene, but were more likely accounted for by
transcriptional down- regulation and/or post-transcriptional factors. No
correlations between GJIC and known oncogene and tumor suppressor gene
alterations in the human lung carcinoma cells were apparent, suggesting
that other mechanisms down-regulate GJIC in these cells. Since the
neoplastic cell lines exhibited low GJIC (either homologous or
heterologous), this characteristic may be involved in expression of the
neoplastic phenotype.
ARTICLES
Frequent reduction of gap junctional intercellular communication and connexin43 expression in human and mouse lung carcinoma cells
Department of Pathology, Medical College of Ohio, Toledo 43699, USA.
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