Carcinogenesis, Vol 19, 1735-1741, Copyright © 1998 by Oxford University Press
A Tanimoto, CY Kao, CC Chang, Y Sasaguri and R Padmanabhan
Eukaryotic cell cycle progression is regulated by an orderly and sequential
activation of several cyclin-dependent kinases, which phosphorylate key
substrates during this process. p34cdc2, the catalytic subunit of cdc2
kinase, is expressed at the late G1/S boundary and is required for the
G2-->M phase transition. Transactivation of the human cdc2 promoter by
the DNA tumor virus- encoded oncogenic protein SV40 large T antigen is
mediated by induction of a novel 110 kDa CCAAT box binding factor
(CBF/cdc2). To investigate whether induction of CBF/cdc2 is an intrinsic
property of the viral oncoprotein or is a common event during
transformation of normal cells, expression of CBF/cdc2 was analyzed in many
human tumor cell lines and in rodent cells spontaneously transformed or
stably expressing various oncogenes. Our results showed that CBF/cdc2 was
overexpressed in all transformed cells examined, including human 293,
MCF-7, HeLa and HepG2 cells. Moreover, expression of CBF/cdc2 was elevated
in spontaneously transformed rat liver epithelial cells (C4T), but not
detectable in the non-tumorigenic parental (RLE) cells. The elevated levels
of CBF/cdc2 expression in C4T cells correlated well with increased cdc2
mRNA and p34cdc2 levels. CBF/cdc2 was also overexpressed in a rat liver
epithelial cell line (WB) stably transfected with various oncogenes, v-
myc, v-Ha-ras and mutated rat neu and v-src. Using an electrophoretic
mobility shift assay, specific binding of CBF/cdc2 to the CCAAT box motifs
of the human cdc2, cycA and cdc25C promoters was detected, suggesting that
transcription of these cell cycle regulatory genes are coordinately
activated by CBF/cdc2.
ARTICLES
Deregulation of cdc2 gene expression correlates with overexpression of a 110 kDa CCAAT box binding factor in transformed cells
Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66160-7421, USA.
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