Carcinogenesis, Vol 19, 1873-1875, Copyright © 1998 by Oxford University Press
EM van Lieshout and WH Peters
Glutathione S-transferases (GSTs) are a family of enzymes involved in the
detoxification of a wide range of chemicals including chemical carcinogens.
Human cytosolic GSTs are divided into four major classes; alpha, mu, pi and
theta. This study was performed to evaluate the influence of age and gender
on the GST isoenzyme expression and glutathione (GSH) content in
lymphocytes. Blood was collected from 124 healthy controls, which were
divided into age groups of 20-40 years (21 females, 20 males), 40-60 years
(20 females, 21 males) and 60-80 years (20 females, 22 males). Lymphocytes
were isolated by density centrifugation on Histopaque-1077. After
homogenization, cytosolic fractions were isolated. Herein, GST isoenzyme
levels were determined by densitometrical analysis of western blots after
immunodetection with monoclonal antibodies. Total GSH content was
determined by high performance liquid chromatography after conjugation with
monobromobimane. Spearman rank correlation and Wilcoxon rank sum tests were
used for statistical evaluation. Lymphocytic GSTmu and pi levels were not
correlated with age or gender. GSTalpha was not detectable in lymphocytes.
GSH contents were not different in males and females, but decreased with
age in both males and females. In age group 60-80, GSH content was
significantly lower as compared with age groups 20-40 and 40-60 in both
sexes. Since high GSH is an essential factor in the detoxification of many
compounds, these data indicate that the detoxification potential of the
GSH/GST system in lymphocytes may decrease with age in man.
ARTICLES
Age and gender dependent levels of glutathione and glutathione S- transferases in human lymphocytes
Department of Gastroenterology, University Hospital St. Radboud, Nijmegen, The Netherlands.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. J. Tijhuis, P. A. Wark, J. M.M.J.G. Aarts, M. H.P.W. Visker, F. M. Nagengast, F. J. Kok, and E. Kampman GSTP1 and GSTA1 Polymorphisms Interact with Cruciferous Vegetable Intake in Colorectal Adenoma Risk Cancer Epidemiol. Biomarkers Prev., December 1, 2005; 14(12): 2943 - 2951. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Du, H. Bayir, Y. Lai, X. Zhang, P. M. Kochanek, S. C. Watkins, S. H. Graham, and R. S. B. Clark Innate Gender-based Proclivity in Response to Cytotoxicity and Programmed Cell Death Pathway J. Biol. Chem., September 10, 2004; 279(37): 38563 - 38570. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J Lenton, A. T Sane, H. Therriault, A. M Cantin, H. Payette, and J R. Wagner Vitamin C augments lymphocyte glutathione in subjects with ascorbate deficiency Am. J. Clinical Nutrition, January 1, 2003; 77(1): 189 - 195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. P. Perera, L. A. Mooney, M. Stampfer, D. H. Phillips, D. A. Bell, A. Rundle, S. Cho, W.-Y. Tsai, J. Ma, A. Blackwood, et al. Associations between carcinogen-DNA damage, glutathione S-transferase genotypes, and risk of lung cancer in the prospective Physicians' Health Cohort Study Carcinogenesis, October 1, 2002; 23(10): 1641 - 1646. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Sharma, H. R. McLelland, K. A. Hill, C. R. Ireson, S. A. Euden, M. M. Manson, M. Pirmohamed, L. J. Marnett, A. J. Gescher, and W. P. Steward Pharmacodynamic and Pharmacokinetic Study of Oral Curcuma Extract in Patients with Colorectal Cancer Clin. Cancer Res., July 1, 2001; 7(7): 1894 - 1900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Lampe, C. Chen, S. Li, J. Prunty, M. T. Grate, D. E. Meehan, K. V. Barale, D. A. Dightman, Z. Feng, and J. D. Potter Modulation of Human Glutathione S-Transferases by Botanically Defined Vegetable Diets Cancer Epidemiol. Biomarkers Prev., August 1, 2000; 9(8): 787 - 793. [Abstract] [Full Text]
|
|
|
|
|
|
G. Quievryn and A. Zhitkovich Loss of DNA-protein crosslinks from formaldehyde-exposed cells occurs through spontaneous hydrolysis and an active repair process linked to proteosome function Carcinogenesis, August 1, 2000; 21(8): 1573 - 1580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J Lenton, H. Therriault, A. M Cantin, T. Fulop, H. Payette, and J R. Wagner Direct correlation of glutathione and ascorbate and their dependence on age and season in human lymphocytes Am. J. Clinical Nutrition, May 1, 2000; 71(5): 1194 - 1200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Rahman, E. Swarska, M. Henry, J. Stolk, and W. MacNee Is there any relationship between plasma antioxidant capacity and lung function in smokers and in patients with chronic obstructive pulmonary disease? Thorax, March 1, 2000; 55(3): 189 - 193. [Abstract] [Full Text]
|
|