Carcinogenesis, Vol 19, 1889-1894, Copyright © 1998 by Oxford University Press
ZQ Zhang, W Zhang, NQ Wang, M Bani-Yaghoub, ZX Lin and CC Naus
The human lung carcinoma cell line PG is defective in gap junctional
intercellular communication (GJIC). Connexin43 (Cx43) mRNA, which is
expressed in normal human lung cells, is undetectable in these tumor cells.
To explore if up-regulation of Cx43 gene expression will suppress
malignancy of PG cells, Cx43 cDNA was co-transfected with pSV2neo cDNA into
PG cells. Control cells were transfected with the blank vector plus neo
cDNA. Several stable Cx43 transfectant clones, which acquired high levels
of Cx43 expression and the capacity of GJIC, were compared with control
clones and the parental cell line, both of which lacked Cx43 expression and
GJIC. The control clones resembled the parental cells in exhibiting high
cell growth rate, weak attachment to the substratum and a high frequency of
colony formation in soft agar. In contrast to the control cells, Cx43
transfected clones showed reduced growth rate, enhanced attachment to the
substratum and inhibition of colony formation in soft agar. In vivo results
from nude mice experiments showed high tumorigenicity with control clones
and inhibition of tumorigenicity in Cx43 transfected clones. The
consistency between in vitro and in vivo results strongly suggests a tumor
suppressing effect of the Cx43 gene in human lung carcinoma cells.
ARTICLES
Suppression of tumorigenicity of human lung carcinoma cells after transfection with connexin43
Beijing Institute for Cancer Research, School of Oncology, Beijing Medical University.
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