Carcinogenesis, Vol 19, 1913-1917, Copyright © 1998 by Oxford University Press
A Rothfuss, C Dennog and G Speit
Hyperbaric oxygen (HBO) treatment (i.e. exposure to 100% oxygen at a
pressure of 2.5 ATA for a total of three 20 min periods) of human subjects
caused clear and reproducible DNA effects in the comet assay with
leukocytes. Interestingly, DNA damage was detected only after the first
treatment and not after further treatments under the same conditions,
indicating an increase in antioxidant defences. We now demonstrate that
blood taken 24 h after HBO treatment is well protected against the in vitro
induction of DNA damage by hydrogen peroxide (H2O2). H2O2 treatment caused
a significant induction of DNA effects in the comet assay and chromosome
breakage in the micronucleus test in the blood of volunteers before HBO.
The same treatment did not cause genotoxic effects 24 h after HBO. This
protective effect lasted for at least 1 week. Experiments with isolated
lymphocytes gave similar results, indicating that the adaptive response is
a cellular effect. The cells were not comparably protected against the
genotoxic effects of gamma-irradiation, suggesting increased scavenging of
reactive oxygen species distant from nuclear DNA.
ARTICLES
Adaptive protection against the induction of oxidative DNA damage after hyperbaric oxygen treatment
Universitatsklinikum Ulm, Abteilung Medizinische Genetik, Germany.
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