Carcinogenesis, Vol 19, 281-285, Copyright © 1998 by Oxford University Press
IR Rowland, CJ Rumney, JT Coutts and LC Lievense
The effect of Bifidobacterium longum (4 x 10(8) viable cells/g diet) and a
derivative of inulin ('Raftiline HP'; 5% w/w in diet) on colonic aberrant
crypt foci (ACF) induced by the colon carcinogen azoxymethane (AOM) has
been studied. The concentration of ammonia, a putative tumour promoter
produced by bacterial degradation of protein and urea, and the activities
of certain bacterial enzymes thought to play a role in colon
carcinogenesis, beta-glucuronidase and beta-glucosidase were also assayed.
Consumption of either B. longum or inulin was associated with a decrease
(26 and 41%, respectively) in AOM-induced small ACF (i.e. those comprising
1-3 aberrant crypts per focus). Combined administration of the
bifidobacterium and inulin resulted in more potent inhibition of ACF than
administration of the two separately, achieving 80% inhibition of small
ACF. Furthermore, the combined administration significantly decreased the
incidence (by 59%) of large ACF (>4 aberrant crypts per focus), which
are considered to be predictive of eventual tumour incidence. Since the
dietary treatments were started 1 week after the carcinogen dose, the
results suggest that B. longum and inulin may be affecting the early
promotion phase of the carcinogenic process. Consumption of diets
containing B. longum, inulin or both were also associated with decreases in
beta-glucuronidase activity and ammonia concentration in the caecal
contents. Both these factors have been associated with carcinogenesis of
the colon in experimental animal models. In rats given inulin-containing
diets (with or without B. longum) an increase in caecal wt and
beta-glucosidase activity and a decrease in caecal pH were observed. The
results suggest that consumption of B. longum or inulin was associated with
potentially beneficial changes in caecal physiology and bacterial metabolic
activity in relation to tumour risk and in the incidence of putative
preneoplastic lesions in the colon. The results also indicated that
combined treatment with the two agents was more effective in reducing
colonic lesions.
ARTICLES
Effect of Bifidobacterium longum and inulin on gut bacterial metabolism and carcinogen-induced aberrant crypt foci in rats
Northern Ireland Centre for Diet and Health, School of Biomedical Sciences, University of Ulster, Coleraine, UK. I.Rowland@ulst.ac.uk
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. A. Barry, D. C. Hernot, I. S. Middelbos, C. Francis, B. Dunsford, K. S. Swanson, and G. C. Fahey Jr. Low-level fructan supplementation of dogs enhances nutrient digestion and modifies stool metabolite concentrations, but does not alter fecal microbiota populations J Anim Sci, October 1, 2009; 87(10): 3244 - 3252. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-J. Lee, R. I. Mackie, I. K. O. Cann, and J. Wiegel Description of Caldanaerobius fijiensis gen. nov., sp. nov., an inulin-degrading, ethanol-producing, thermophilic bacterium from a Fijian hot spring sediment, and reclassification of Thermoanaerobacterium polysaccharolyticum and Thermoanaerobacterium zeae as Caldanaerobius polysaccharolyticus comb. nov. and Caldanaerobius zeae comb. nov. Int J Syst Evol Microbiol, March 1, 2008; 58(3): 666 - 670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Humblot, M. Murkovic, L. Rigottier-Gois, M. Bensaada, A. Bouclet, C. Andrieux, J. Anba, and S. Rabot {beta}-Glucuronidase in human intestinal microbiota is necessary for the colonic genotoxicity of the food-borne carcinogen 2-amino-3-methylimidazo[4,5-f]quinoline in rats Carcinogenesis, November 1, 2007; 28(11): 2419 - 2425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rafter, M. Bennett, G. Caderni, Y. Clune, R. Hughes, P. C Karlsson, A. Klinder, M. O'Riordan, G. C O'Sullivan, B. Pool-Zobel, et al. Dietary synbiotics reduce cancer risk factors in polypectomized and colon cancer patients Am. J. Clinical Nutrition, February 1, 2007; 85(2): 488 - 496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Ewaschuk, J. W. Walker, H. Diaz, and K. L. Madsen Bioproduction of Conjugated Linoleic Acid by Probiotic Bacteria Occurs In Vitro and In Vivo in Mice J. Nutr., June 1, 2006; 136(6): 1483 - 1487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Le Leu, I. L. Brown, Y. Hu, A. R. Bird, M. Jackson, A. Esterman, and G. P. Young A Synbiotic Combination of Resistant Starch and Bifidobacterium lactis Facilitates Apoptotic Deletion of Carcinogen-Damaged Cells in Rat Colon J. Nutr., May 1, 2005; 135(5): 996 - 1001. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Rosberg-Cody, R. P. Ross, S. Hussey, C. A. Ryan, B. P. Murphy, G. F. Fitzgerald, R. Devery, and C. Stanton Mining the Microbiota of the Neonatal Gastrointestinal Tract for Conjugated Linoleic Acid-Producing Bifidobacteria Appl. Envir. Microbiol., August 1, 2004; 70(8): 4635 - 4641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Femia, C. Luceri, P. Dolara, A. Giannini, A. Biggeri, M. Salvadori, Y. Clune, K. J. Collins, M. Paglierani, and G. Caderni Antitumorigenic activity of the prebiotic inulin enriched with oligofructose in combination with the probiotics Lactobacillus rhamnosus and Bifidobacterium lactis on azoxymethane-induced colon carcinogenesis in rats Carcinogenesis, November 1, 2002; 23(11): 1953 - 1960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. A. Apajalahti, H. Kettunen, A. Kettunen, W. E. Holben, P. H. Nurminen, N. Rautonen, and M. Mutanen Culture-Independent Microbial Community Analysis Reveals that Inulin in the Diet Primarily Affects Previously Unknown Bacteria in the Mouse Cecum Appl. Envir. Microbiol., October 1, 2002; 68(10): 4986 - 4995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Verghese, D. R. Rao, C. B. Chawan, and L. Shackelford Dietary Inulin Suppresses Azoxymethane-Induced Preneoplastic Aberrant Crypt Foci in Mature Fisher 344 Rats J. Nutr., September 1, 2002; 132(9): 2804 - 2808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Verghese, D. R. Rao, C. B. Chawan, L. L. Williams, and L. Shackelford Dietary Inulin Suppresses Azoxymethane-Induced Aberrant Crypt Foci and Colon Tumors at the Promotion Stage in Young Fisher 344 Rats J. Nutr., September 1, 2002; 132(9): 2809 - 2813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Duggan, J. Gannon, and W A. Walker Protective nutrients and functional foods for the gastrointestinal tract Am. J. Clinical Nutrition, May 1, 2002; 75(5): 789 - 808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Tavan, C. Cayuela, J.-M. Antoine, G. Trugnan, C. Chaugier, and P. Cassand Effects of dairy products on heterocyclic aromatic amine-induced rat colon carcinogenesis Carcinogenesis, March 1, 2002; 23(3): 477 - 483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Buddington, J. B. Donahoo, and R. K. Buddington Dietary Oligofructose and Inulin Protect Mice from Enteric and Systemic Pathogens and Tumor Inducers J. Nutr., March 1, 2002; 132(3): 472 - 477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Takagi, T. Matsuzaki, M. Sato, K. Nomoto, M. Morotomi, and T. Yokokura Enhancement of natural killer cytotoxicity delayed murine carcinogenesis by a probiotic microorganism Carcinogenesis, April 1, 2001; 22(4): 599 - 605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B Roberfroid Prebiotics: preferential substrates for specific germs? Am. J. Clinical Nutrition, February 1, 2001; 73(2): 406S - 409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Wollowski, G. Rechkemmer, and B. L Pool-Zobel Protective role of probiotics and prebiotics in colon cancer Am. J. Clinical Nutrition, February 1, 2001; 73(2): 451S - 455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Hughes and I.R. Rowland Stimulation of apoptosis by two prebiotic chicory fructans in the rat colon Carcinogenesis, January 1, 2001; 22(1): 43 - 47. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. R. Bruce, A. Giacca, and A. Medline Possible Mechanisms Relating Diet and Risk of Colon Cancer Cancer Epidemiol. Biomarkers Prev., December 1, 2000; 9(12): 1271 - 1279. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. E. George, G. M. Nelson, A. E. Swank, L. R. Brooks, K. Bailey, M. George, and A. DeAngelo The Disinfection By-Products Dichloro-, Dibromo-, and Bromochloroacetic Acid Impact Intestinal Microflora and Metabolism in Fischer 344 Rats upon Exposure in Drinking Water Toxicol. Sci., August 1, 2000; 56(2): 282 - 289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B Roberfroid Prebiotics and probiotics: are they functional foods? Am. J. Clinical Nutrition, June 1, 2000; 71(6): 1682S - 1687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Brady, D. D. Gallaher, and F. F. Busta The Role of Probiotic Cultures in the Prevention of Colon Cancer J. Nutr., February 1, 2000; 130(2): 410 - 410. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Parnaud, S. Tache, G. Peiffer, and D. E. Corpet Polyethylene-glycol Suppresses Colon Cancer and Causes Dose-dependent Regression of Azoxymethane-induced Aberrant Crypt Foci in Rats Cancer Res., October 1, 1999; 59(20): 5143 - 5147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Pierre, P. Perrin, E. Bassonga, F. Bornet, K. Meflah, and J. Menanteau T cell status influences colon tumor occurrence in Min mice fed short chain fructo-oligosaccharides as a diet supplement Carcinogenesis, October 1, 1999; 20(10): 1953 - 1956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Roberfroid Concepts in Functional Foods: The Case of Inulin and Oligofructose J. Nutr., July 1, 1999; 129(7): 1398 - 1398. [Abstract] [Full Text]
|
|
|
|
|
|
K. R. Niness Inulin and Oligofructose: What Are They? J. Nutr., July 1, 1999; 129(7): 1402 - 1402. [Abstract] [Full Text]
|
|
|
|
|
|
D. D. Gallaher and J. Khil The Effect of Synbiotics on Colon Carcinogenesis in Rats J. Nutr., July 1, 1999; 129(7): 1483 - 1483. [Abstract] [Full Text]
|
|
|
|
|
|
D. E. Corpet and G. Parnaud Polyethylene-glycol, a potent suppressor of azoxymethane-induced colonic aberrant crypt foci in rats Carcinogenesis, May 1, 1999; 20(5): 915 - 918. [Abstract] [Full Text] [PDF]
|
|