Carcinogenesis, Vol 19, 353-357, Copyright © 1998 by Oxford University Press
G Flamini, G Romano, G Curigliano, A Chiominto, G Capelli, A Boninsegna, C Signorelli, L Ventura, RM Santella, A Sgambato and A Cittadini
4-Aminobiphenyl (4-ABP)-DNA adducts and p53 overexpression were evaluated
in laryngeal biopsies from 38 patients by immunohistochemical methods.
Samples were categorized as tumors (n = 9), polyps (n = 28) or normal
tissue (n = 1). 4-ABP-DNA adducts were evaluated with a quantitative
immunoperoxidase method using monoclonal antibody 3C8 in both the lesion
and adjacent tissue. Relative staining intensity data showed a log-normal
distribution and values found in adjacent tissue from smokers were
significantly higher (median: 173.5, geometric mean: 159.9) than those
measured in adjacent tissue from non-smokers (median: 75.5, geometric mean:
7.40). Statistical significance was assessed both by non-parametric testing
on raw data (P = 0.0007 on rank sum test) and by parametric testing on
log-transformed data (P = 0.0002 on an unpaired t-test). Furthermore,
relative staining intensity in the lesional tissue showed the same
significant difference between smokers and non-smokers in patients affected
by polyps, whereas no significant difference was detected in patients with
laryngeal tumors. Overexpression of p53, also measured with an
immunoperoxidase method, was observed in 44% of the malignant tumors and in
3.5% of the polyps. This work demonstrates that 4-ABP-DNA adducts can be
evaluated in laryngeal tissue and are related to smoking exposure.
ARTICLES
4-Aminobiphenyl-DNA adducts in laryngeal tissue and smoking habits: an immunohistochemical study
Institute of General Pathology-Centro di Ricerche Oncologiche Giovanni XXIII, Catholic University School of Medicine, Largo F. Vito, Rome, Italy. ibipg@rm.unicatt.it
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