Carcinogenesis, Vol 19, 525-527, Copyright © 1998 by Oxford University Press
T Izumi and S Mitra
AP-endonuclease (APE) plays a central role in the base excision repair
process for oxidative and alkylation damage in DNA. The major human APE
with 318 amino acid residues possesses transcriptional regulatory
activities for which the N-terminal region was found to be essential.
Systematic deletion studies of both amino and carboxyl termini of the human
APE have shown that the carboxyl termini residues are essential for the
endonuclease activity as indicated by direct measurement of enzyme activity
and from studies on phenotypic rescue of Escherichia coli. However, the
amino-terminal residues are dispensable for this activity and the boundary
of the active endonuclease lies at this end between positions 61 and 80.
ARTICLES
Deletion analysis of human AP-endonuclease: minimum sequence required for the endonuclease activity
Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77555-1079, USA.
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