Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Liu, Y.
Right arrow Articles by Kulesz-Martin, M. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, Y.
Right arrow Articles by Kulesz-Martin, M. F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Carcinogenesis, Vol 19, 683-686, Copyright © 1998 by Oxford University Press

ARTICLES

Altered gene expression in a clonal epidermal cell model of carcinogenesis identified by RNA differential display

Y Liu and MF Kulesz-Martin
Program of Biochemistry and Department of Experimental Therapeutics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.

We have developed a multistage model system in which a normal mouse keratinocyte clone has been initiated with 7,12- dimethylbenz[a]anthracene and variant clones derived with benign or malignant phenotypes. To identify specific genes altered during mouse skin carcinogenesis, the gene expression patterns of the normal parental epidermal cell, an initiated cell, a benign papilloma, and a poorly differentiated squamous cell carcinoma were compared using RNA differential display. Most alterations in gene expression were observed at malignant conversion, that is, in the poorly differentiated squamous cell carcinoma that is known to have deregulated expression of p53. The sequence of a cloned cDNA fragment lost in the poorly differentiated squamous cell carcinoma was nearly identical to the 3' region of an adhesion-related kinase which is involved in homophilic cell aggregation. It is found in normal epidermal progenitor cells as well as tumorigenic cells with differentiation potential, but not in tumorigenic cells with a poorly differentiated phenotype, suggesting that this adhesion-related kinase may be involved in epidermal cell differentiation. Differential display within the cloned epidermal cell model appears to be useful in detecting and identifying malignant conversion-associated genes which then can be tested directly for their potential role in epithelial carcinogenesis.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 CarcinogenesisHome page
E. J. Horn, A. Albor, Y. Liu, S. El-Hizawi, G. E. Vanderbeek, M. Babcock, G. T. Bowden, H. Hennings, G. Lozano, W. C. Weinberg, et al.
RING protein Trim32 associated with skin carcinogenesis has anti-apoptotic and E3-ubiquitin ligase properties
Carcinogenesis, February 1, 2004; 25(2): 157 - 167.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
Z. Wang, Y. Liu, M. Mori, and M. Kulesz-Martin
Gene expression profiling of initiated epidermal cells with benign or malignant tumor fates
Carcinogenesis, April 1, 2002; 23(4): 635 - 643.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. L. Cmarik, H. Min, G. Hegamyer, S. Zhan, M. Kulesz-Martin, H. Yoshinaga, S. Matsuhashi, and N. H. Colburn
Differentially expressed protein Pdcd4 inhibits tumor promoter-induced neoplastic transformation
PNAS, November 23, 1999; 96(24): 14037 - 14042.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.