Carcinogenesis, Vol 19, 841-846, Copyright © 1998 by Oxford University Press
B Schoket, DH Phillips, S Kostic and I Vincze
Relationships between smoking status and levels of bulky DNA adducts were
investigated in bronchial tissue of lung patients in relation to their
GSTM1 and CYP1A1 MspI genotypes. A total of 150 Hungarian patients
undergoing pulmonary surgery were included in the study, 124 with lung
malignancies and 26 with non-malignant lung conditions. There were
significant relationships between smoking status and bulky DNA adduct
levels, as determined by 32P-post-labelling analysis, in macroscopically
normal bronchial tissues. There was a highly significant difference in the
adduct levels of a combined group consisting of current smokers and
short-term ex-smokers (< or = 1 year abstinence) compared with life-time
non-smokers and long-term ex- smokers (> 1 year abstinence) (P =
0.0001). The apparent half-life was estimated to be 1.7 years for bulky DNA
adducts in the bronchial tissue from ex-smokers. There were no
statistically significant correlations between (i) daily cigarette dose and
DNA adduct levels in current smokers, (ii) DNA adduct level and
histological type of lung cancer, or (iii) GSTM1 and CYP1A1 MspI genotypes
and DNA adduct levels after adjustment for either smoking status or
malignancy. By multiple logistic regression analysis, smoking and GSTM1
null genotype were found to be risk factors for squamous cell carcinoma.
However, bulky DNA adduct levels in bronchial tissue did not appear to be a
statistically-significant risk factor for the major histological types of
lung cancer.
ARTICLES
Smoking-associated bulky DNA adducts in bronchial tissue related to CYP1A1 MspI and GSTM1 genotypes in lung patients
Department of Biochemistry, Johan Bela National Institute of Public Health, Budapest, Hungary. schoket@oki1.joboki.hu
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Anna, R. Holmila, K. Kovacs, E. Gyorffy, Z. Gyori, J. Segesdi, J. Minarovits, I. Soltesz, S. Kostic, A. Csekeo, et al. Relationship between TP53 tumour suppressor gene mutations and smoking-related bulky DNA adducts in a lung cancer study population from Hungary Mutagenesis, November 1, 2009; 24(6): 475 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Taioli Gene-environment interaction in tobacco-related cancers Carcinogenesis, August 1, 2008; 29(8): 1467 - 1474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Schwartz, G. M. Prysak, C. H. Bock, and M. L. Cote The molecular epidemiology of lung cancer Carcinogenesis, March 1, 2007; 28(3): 507 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Gyorffy, L. Anna, Z. Gyori, J. Segesdi, J. Minarovits, I. Soltesz, S. Kostic, A. Csekeo, M. C. Poirier, and B. Schoket DNA adducts in tumour, normal peripheral lung and bronchus, and peripheral blood lymphocytes from smoking and non-smoking lung cancer patients: correlations between tissues and detection by 32P-postlabelling and immunoassay Carcinogenesis, July 1, 2004; 25(7): 1201 - 1209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Hung, P. Boffetta, J. Brockmoller, D. Butkiewicz, I. Cascorbi, M. L. Clapper, S. Garte, A. Haugen, A. Hirvonen, S. Anttila, et al. CYP1A1 and GSTM1 genetic polymorphisms and lung cancer risk in Caucasian non-smokers: a pooled analysis Carcinogenesis, May 1, 2003; 24(5): 875 - 882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Alexandrov, I. Cascorbi, M. Rojas, G. Bouvier, E. Kriek, and H. Bartsch CYP1A1 and GSTM1 genotypes affect benzo[a]pyrene DNA adducts in smokers' lung: comparison with aromatic/hydrophobic adduct formation Carcinogenesis, December 1, 2002; 23(12): 1969 - 1977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Phillips Smoking-related DNA and protein adducts in human tissues Carcinogenesis, December 1, 2002; 23(12): 1979 - 2004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Godschalk, J. Nair, F.J. van Schooten, A. Risch, P. Drings, K. Kayser, H. Dienemann, and H. Bartsch Comparison of multiple DNA adduct types in tumor adjacent human lung tissue: effect of cigarette smoking Carcinogenesis, December 1, 2002; 23(12): 2081 - 2086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. G. Shields Tobacco Smoking, Harm Reduction, and Biomarkers J Natl Cancer Inst, October 2, 2002; 94(19): 1435 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Garte, L. Gaspari, A.-K. Alexandrie, C. Ambrosone, H. Autrup, J. L. Autrup, H. Baranova, L. Bathum, S. Benhamou, P. Boffetta, et al. Metabolic Gene Polymorphism Frequencies in Control Populations Cancer Epidemiol. Biomarkers Prev., December 1, 2001; 10(12): 1239 - 1248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. B. J. Smith, P. A. Harper, J. M. Y. Wong, M. S. M. Lam, K. R. Reid, D. Petsikas, and T. E. Massey Human Lung Microsomal Cytochrome P4501A1 (CYP1A1) Activities: Impact of Smoking Status and CYP1A1, Aryl Hydrocarbon Receptor, and Glutathione S-Transferase M1 Genetic Polymorphisms Cancer Epidemiol. Biomarkers Prev., August 1, 2001; 10(8): 839 - 853. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Song, W. Tan, D. Xing, and D. Lin CYP 1A1 polymorphism and risk of lung cancer in relation to tobacco smoking: a case-control study in China Carcinogenesis, January 1, 2001; 22(1): 11 - 16. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rundle, D. Tang, J. Zhou, S. Cho, and F. Perera The Association between Glutathione S-Transferase M1 Genotype and Polycyclic Aromatic Hydrocarbon-DNA Adducts in Breast Tissue Cancer Epidemiol. Biomarkers Prev., October 1, 2000; 9(10): 1079 - 1085. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. C. Povey DNA Adducts: Endogenous and Induced Toxicol Pathol, May 1, 2000; 28(3): 405 - 414. [Abstract] [PDF]
|
|
|
|
|
|
H. Bartsch, U. Nair, A. Risch, M. Rojas, H. Wikman, and K. Alexandrov Genetic Polymorphism of CYP Genes, Alone or in Combination, as a Risk Modifier of Tobacco-related Cancers Cancer Epidemiol. Biomarkers Prev., January 1, 2000; 9(1): 3 - 28. [Abstract] [Full Text]
|
|
|
|
|
|
M. Rojas, I. Cascorbi, K. Alexandrov, E. Kriek, G. Auburtin, L. Mayer, A. Kopp-Schneider, I. Roots, and H. Bartsch Modulation of benzo[a]pyrene diolepoxide-DNA adduct levels in human white blood cells by CYP1A1, GSTM1 and GSTT1 polymorphism Carcinogenesis, January 1, 2000; 21(1): 35 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Izzotti, M. Bagnasco, F. D'Agostini, C. Cartiglia, R. A. Lubet, G. J Kelloff, and S. De Flora Formation and persistence of nucleotide alterations in rats exposed whole-body to environmental cigarette smoke Carcinogenesis, August 1, 1999; 20(8): 1499 - 1506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mollerup, D. Ryberg, A. Hewer, D. H. Phillips, and A. Haugen Sex Differences in Lung CYP1A1 Expression and DNA Adduct Levels among Lung Cancer Patients Cancer Res., July 1, 1999; 59(14): 3317 - 3320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ozawa, B. Schoket, L.P. McDaniel, Y.-M. Tang, C. B. Ambrosone, S. Kostic, I. Vincze, and F. F. Kadlubar Analyses of bronchial bulky DNA adduct levels and CYP2C9, GSTP1 and NQO1 genotypes in a Hungarian study population with pulmonary diseases Carcinogenesis, June 1, 1999; 20(6): 991 - 995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Spitz, M. de Andrade, and J. Di Giovanni Molecular Dosimeters of Smoking Damage in the Lung J Natl Cancer Inst, April 7, 1999; 91(7): 578 - 579. [Full Text] [PDF]
|
|